诊断敏感实验琼脂 英文名称: Diagnostic Sensitivity Test Agar | ||||||||||||||||||||||||||||||||||||||||||||||
型号:null 产品货号: CM0261B | ||||||||||||||||||||||||||||||||||||||||||||||
价格:请致电:010-57128832,18610462672 | ||||||||||||||||||||||||||||||||||||||||||||||
品牌: 英国 | ||||||||||||||||||||||||||||||||||||||||||||||
DIAGNOSTIC SENSITIVITY TEST AGAR (DST AGAR) Code: CM0261 A susceptibility test agar for antimicrobial testing.
* Adjusted as required to meet performance standardsDirections Description The diagnostic role was supported by the nutritional amino-acid base with glucose to encourage early growth. The inclusion of the buffers (disodium phosphate and sodium acetate) helped prevent excessive movements of pH values which could result from utilisation of glucose or amino-acids. Such pH movements would interfere with haemolytic reactions1 and the MIC values of pH-susceptible antimicrobials2. Aneurine, added as a general purpose vitamin, improved the growth of several organisms especially staphylococci. The agar used in the formulation has been specially processed to allow unimpeded diffusion of antimicrobials from discs3. An essential requirement for satisfactory antimicrobial susceptibility media is that the reactive levels of thymidine and thymine must be sufficiently reduced to avoid antagonism of trimethoprim and sulphonamides4. DSTA meets this requirement and in the presence of lysed horse blood (or defibrinated horse blood if the plates are stored long enough to allow some lysis of the erythrocytes) the level of thymidine will be further reduced. This is caused by the action of the enzyme thymidine phosphorylase which is released from lysed horse erythrocytes5. Thymidine is an essential growth factor for thymidine-dependent organisms and they will not grow in its absence or they will grow poorly in media containing reduced levels6. It is important that users of DSTA are aware of this limitation of thymidine which now exists in the medium and the effect it will have on a small proportion of organisms. Details of the function of the medium and the methodology used for antimicrobial susceptibility tests are discussed in the Section `Susceptibility Testing’. Storage conditions and Shelf life Appearance Quality control
* This organism is available as a Culti-Loop® |