MILK PLATE COUNT AGAR
PLATE COUNT AGAR WITH ANTIBIOTIC FREE SKIM MILK
Code: CM0681
A medium for the enumeration of viable organisms in milk and dairy products.
Typical Formula* | gm/litre |
Tryptone | 5.0 |
Yeast extract | 2.5 |
Glucose | 1.0 |
Antibiotic free skim milk | 1.0 |
Agar | 10.0 |
pH 6.9 ± 0.1 @ 25°C | |
* Adjusted as required to meet performance standards
Directions
Suspend 19.5g in 1 litre of distilled water. Bring to the boil with frequent agitation, mix and distribute into final containers. Sterilise by autoclaving at 121°C for 15 minutes.
Description
Plate Count Agar with Antibiotic Free Skim Milk is equivalent to the medium recommended by British Standards Institution1 and International Organization for Standardization2. The medium is used for the enumeration of viable organisms in milk and dairy products.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30°C and use before the expiry date on the label.
Store the prepared plates at 2-8°C.
Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Straw coloured gel
Quality control
Positive control: | Expected results |
| Escherichia coli ATCC® 25922* | Good growth; straw coloured colonies |
Negative control: | |
Uninoculated medium | No change |
* This organism is available as a Culti-Loop®
Compare with previous lot/batch using pasteurised and raw milk samples, incubated at 32-35°C for 48 hours.
Precautions
Make sure that the procedures and media used in milk product testing comply with the National Regulations required for each country.