| beta actin | |||||||||||||||||||||||||||||||||||
| 型号:null 抗体货号： | |||||||||||||||||||||||||||||||||||
| 价格:请致电：010-57128832,18610462672 | |||||||||||||||||||||||||||||||||||
| 品牌: 美国 产品商标: bioworld | |||||||||||||||||||||||||||||||||||
β-Actin pAb
Product1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2. Molecular Weight~ 45 kDa Purification & PurityThe pAb was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Specificityβ-Actin pAb detects endogenous levels of β-Actin protein. Applications (Recommended Dilutions)WB:1:3000-1:10000 Western Blot(WB) Western blot (WB) analyzes of β-Actin pAb in extracts from Rat brain/Mouse raw cell/Hela cells. Storage&StabilityStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. BackgroundAll eukaryotic cells express actin, which often constitutes asmuch as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of actin are present in mammalian tissues and fall into three classes. -actin expression is limited to various types of muscle, whereas – and -actin are the principle constituents of filaments in other tissues.Members of the small GTPase family regulate the organization of the actin cytoskeleton. Rho controls the assembly of ctin stress fibers and focal adhesion, Rac regulates ctin filament accumulation at the plasma membrane and Cdc42 stimulates formation of filopodia. | |||||||||||||||||||||||||||||||||||
