Product
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
Purification & Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Specificity
Tubulin beta 1/2/3/5/6/8 pAb pAb detects endogenous levels of Tubulin beta 1/2/3/5/6/8 pAb protein.
Applications (Recommended Dilutions)
WB:1:5000-1:10000
Western Blot(WB)
Western blot (WB) analyzes of Tubulin beta 1/2/3/5/6/8 pAb in extracts from hela cells, mouse brain and rat liver tissues .
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Background
Tubulin is the major building block of microtubules. This intracellular cylindrical filamentous structure is present in almost all eukaryotic cells. They consist principally of 2 soluble proteins, alpha- and beta-tubulin. Microtubules function as structural and mobile elements in mitosis, intracellular transport, flagellar movement, and in the cytoskeleton. Beta Tubulin is a candidate gene for the microtubule dysfunction that occurs in the immotile cilia syndrome.