This KAPA Library Preparation Kit is designed for
the preparation of libraries for sequencing on the Ion
Personal Genome Machine™ (PGM™) and Ion Proton™
semiconductor sequencers. The kit provides all of the
enzymes and reaction buffers required for constructing
libraries from 100 ng – 1 μg of fragmented, doublestranded
DNA via the following steps:
1. End repair: Produce blunt-ended, 5-phosphorylated
fragments.
2. Adapter ligation and nick repair: Ligate dsDNA
adapters to blunt-ended library fragments and
perform nick repair to yield library fragments flanked
by adapters.
3. Library amplification (optional): Perform PCR to
amplify library fragments carrying appropriate adapter
sequences on both ends.

 

Product Applications
The KAPA Library Preparation Kit for Ion Torrent™ Platforms
is ideally suited for NGS library construction workflows
that involve end repair, adapter ligation and nick repair,
and library amplification (optional). The protocol may be
adapted for incorporation into workflows for a wide range
of NGS applications, including:
• Targeted sequencing by solution hybrid selection
• Amplicon sequencing
• Whole-genome shotgun sequencing
• RNA-seq
• ChIP-seq

KAPA Library Preparation Kit Ion Torrent Platforms TDS.pdf