Features

·               LiveWare includes host cells and plasmids that contain chimeras to enable G_αs, G_αi, G_αo, or G_αz coupled GPCR receptors to signal through calcium mobilization and take advantage of the high throughput capabilities of FLIPR Calcium Assay Kits and the FLIPR^TETRA System.  

·               LiveWare reagents have been successfully used on the FLIPR System, in conjunction with non-G_q-coupled members of chemokine, serotonin, glutamate, dopamine, opoid, vasopressin and a- and b-adrenoceptor receptor families. 

·               Receptor configuration options are increased as LiveWare products are available in the following configurations (plasmids are available as hygromycin and neomycin (G418)-resistant; cells are hygromycin-resistant*:

·               Host cells: CHO cells stably transfected with one of the G-protein chimera genes or the G_α16 gene.

·               Plasmids: Replicating G-protein expression vectors. 

*Each plasmid order contains one vial of hygromycin-resistance plasmid and one vial of neomycin (G418)-resistance plasmid.

 

Specifications

Information about LiveWare cells and plasmids is listed in the chart below. Click on the Product # to view a product information sheets.  Vector maps and sequences are available by request from technical support or your sales representative.

LiveWare Product Specifications

Product #	Description
Host Cells
RD-HGA16	Ga16-coupled CHO Cells
RD-HGQO5	Gqo5-coupled CHO Cells
RD-HGQS5	Gqs5-coupled CHO Cells
RD-HGQS5 (G418)	Gqs5-coupled CHO Cells
RD-HGQZ5	Gqz5-coupled CHO Cells
RD-HGQZ5 (G418)	Gqz5 coupled CHO Cells
Contains plasmids for 2 selection antibiotics
RD-PGA16	Plasmids containing Ga16 Gene
RD-PGQI5	Plasmids containing Gqi5 Gene
RD-PGQO5	Plasmids containing Gqo5 Gene
RD-PGQS5	Plasmids containing Gqs5 Gene
RD-PGQZ5	Plasmids containing Gqz5 Gene

For information about sequences or vector maps,  please contact Technical Support or your Sales Representative.