Endogen offers a broad range of recombinant cytokines, chemokines, growth factors and active kinases for bovine, canine, equine, feline, human, mouse, porcine, primate, rat and Rhesus monkey. All of Endogen’s proteins are fully validated and QC-tested (N-terminal sequence, bioassay and SDS-PAGE analysis) to ensure high biological activity and accurate, reproducible results. Applications include cell culture maintenance, bioassays, in vivo research and standards for Western blot and ELISA. Whenever possible, new Endogen recombinant proteins will be expressed in a yeast system, Pichia pastoris, which generates proteins that are secreted, properly folded, and glycosylated in a manner similar to mammalian systems. Most proteins are provided carrier-free and low endotoxin. A lot-specific Certificate of Analysis is provided with each protein.

Species: Feline
Analyte: TNF alpha
Type: Recombinant protein
Activity: The biological activity of recombinant feline TNF a was measured by its cytolytic effect on mouse WEHI-13VAR cells in the presence of Actinomycin D.
Format: Lyophilized, preservative and carrier free
Expression: Mature, soluble form of feline TNF a. GenBank Accession # P19101,
amino acids 77 - 233.
Protein Source: Pichia pastoris
Molecule Weight: 17.3 kDa
Purity: >95%

Homology:

Bovine TNFα	Canine TNFα	Equine TNFα	Feline TNFα	Human TNFα	Mouse TNFα	Ovine TNFα	Porcine TNFα	Rat TNFα	Rhesus macaque TNFα
100	79	79	80.3	80.9	75	92.4	83.3	74.4	80.9	Bovine TNF α
-	100	89.2	95.5	93.6	79.5	79.6	86.5	78.8	94.3	Canine TNFα
-	-	100	89.2	89.2	80.1	77.7	84	78.8	89.2	Equine TNFα
-	-	-	100	93	80.1	80.9	86.5	79.5	93.6	Feline TNFα
-	-	-	-	100	79.5	80.9	87.2	78.8	98.1	Human TNFα
-	-	-	-	-	100	73.7	76.9	94.9	79.5	Mouse TNFα
-	-	-	-	-	-	100	83.3	74.4	80.9	Ovine TNFα
-	-	-	-	-	-	-	100	76.3	87.2	Porcine TNFα
-	-	-	-	-	-	-	-	100	78.8	Rat TNFα
-	-	-	-	-	-	-	-	-	100	Rhesus macaque TNFα

Product #	Description	Pkg. Size
RFTNFAI	Recombinant Feline TNF alpha	20 µg