SulfoLink Coupling Resin is porous, crosslinked, 6% beaded agarose that has been activated with iodoacetyl groups. When incubated with a solution of peptide or protein that contains reduced cysteine residues, the iodoacetyl groups react specifically and efficiently with the exposed sulfhydryls (-SH) to form covalent and irreversible thioether bonds that permanently attach the peptide or protein to the resin. The result is a custom-made affinity resin for purification of antibodies, antigens and other molecules of interest.

Choose one of two convenient SulfoLink Immobilization Kits for peptides or proteins. Each kit contains all the reagents for preparing the sample and five columns containing 2 ml of SulfoLink Resin that can be used in either gravity-flow or centrifuge format for efficient coupling reactions and multiple cycles of affinity purification. A single-column Trial Kit for use with either peptides or proteins is also available, or the SulfoLink Coupling Resin can be purchased separately.

General structure and reaction scheme for SulfoLink Resin

Learn more about the SulfoLink Immobilization Kit for Peptides (Opens a new window)

Learn more about the SulfoLink Immobilization Kit for Proteins

Highlights:

• Specific conjugation through sulfhydryl (-SH) groups – The iodoacetyl groups react specifically with sulhydryls to form irreversible thioether bonds
• Separate kits optimized for peptides or proteins – Kits include optimized reagents for preparing peptide or protein samples for efficient immobilization
• Fast – Spin columns increase protocol speed; prepare and couple samples in 2 hours (peptides) to 3.5 hours (proteins)
• Flexible coupling conditions – Use pH 7.5-9.0 aqueous buffers, organic solvent (e.g., 20% DMSO) or denaturant (guanidine•HCl), as needed for protein or peptide solubility during coupling reaction
• Easy-to-follow instructions – streamlined protocols for sample preparation, immobilization, and affinity purification
• High capacity – Immobilize 1-2 mg peptide or 2-20 mg protein per 2-ml column of SulfoLink Coupling Resin.

Applications:

• Immobilize peptides having terminal cysteine residues to purify antibodies that were generated against peptide immunogens prepared by maleimide conjugation
• Immobilize antibodies in oriented manner through hinge-region sulfhydryls to ensure that antigen binding sites are not sterically hindered for antigen affinity purification

Related Products:

Bond-Breaker TCEP Solution, Neutral pH
Imject Maleimide Activated mcKLH Kit
UltraLink Iodoacetyl Resin
Micro-scale Peptide Coupling Kit
AminoLink Plus Immobilization Kit
CarboLink Immobilization Kit
CarboxyLink Immobilization Kit
Pierce Centrifuge Columns

Product #	Description	Pkg. Size
44995	SulfoLink Immobilization Kit for Proteins Sufficient reagents to prepare five affinity columns. Kit contains: SulfoLink Columns, 5 x 2 ml SulfoLink Sample Preparation Buffer, 7.5 ml SulfoLink Coupling Buffer, 500 ml Wash Solution, 120 ml 2-Mercaptoethylamine•HCl, 5 x 6 mg L-Cysteine•HCl, 100 mg Zeba Desalt Spin Columns, 5 x 5 ml Phosphate Buffered Saline, 1 Pack Columns Accessories Replaces product # 44895 for use with proteins	Kit
44999	SulfoLink Immobilization Kit for Peptides Sufficient reagents to prepare five affinity columns. Kit contains: SulfoLink Columns, 5 x 2 ml SulfoLink Coupling Buffer, 120 ml Wash Solution, 120 ml Bond-Breaker TCEP Solution, 0.5 ml L-Cysteine•HCl, 100 mg Phosphate Buffered Saline, 1 Pack Column Accessories Replaces product # 44895 for use with peptides	Kit
20325	SulfoLink Immobilization Trial Kit Sufficient reagents to prepare 1 affinity column with a peptide or protein. Kit contains: SulfoLink Column, 1 x 2 ml SulfoLink Sample Preparation Buffer, 7.5 ml SulfoLink Coupling Buffer, 120 ml Wash Solution, 25 ml 2-Mercaptoethylamine•HCl, 6 mg Bond-Breaker TCEP Solution, 0.5 ml Cysteine•HCl, 100 mg Zeba Desalt Spin Column, 5 ml Phosphate Buffered Saline, 1 Pack Columns Accessories Replaces product # 20405	Kit
20401	SulfoLink Coupling Resin	10 ml
20402	SulfoLink Coupling Resin	50 ml
20404	SulfoLink Coupling Resin	250 ml