A complete, optimized ELISA kit specific for rat interleukin 6 (IL-6).

The Thermo Scientific Pierce Rat IL-6 ELISA Kit is a colorimetric enzyme-linked immunosorbent assay for quantification of rat interleukin 6 in serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples. The kit features a user-friendly procedure, short assay time, and small sample size – ideal for researchers working with rat serum, plasma or 96-well cell culture experiments.

Each kit lot is carefully tested to ensure optimal performance and consistency from one lot to the next. The kit is offered in single microplate, cost-effective 2-plate, and space-saving 5-plate kit sizes.

 

 

Rat IL-6 ELISA Kit specifications: Assay Range: 31-2,000 pg/ml Sensitivity: less than 16 pg/ml Assay Time: 4 hours Sample Volume: 50 µl per well Format: 96-well strip plate Detection: colorimetric (TMB substrate) Validated Sample Types: culture supernatant, serum and plasma (EDTA, sodium citrate and heparin) Specificity: human IFN gamma. Does not cross-react with the following cytokines: rat GCSF, GM-CSF, IFNg, IL-1a, IL-1b, IL-2, IL-3, IL-4, IL-5, IL-7, IL-9, IL-10, IL-18, Eotaxin, MIP-1a, RANTES and TNFa; or human, mouse and porcine IL-6

Rat IL-6 ELISA protocol. Click on image to obtain complete product instructions including a graphical procedure summary and descriptions of sample preparation and dilution of standards.

 

Convenient ELISA kit package sizes to suit your needs. Components and quantities in each Thermo Scientific Pierce Rat IL-6 ELISA Kit.
Kit Component	1-plate Kit	2-plate Kit	5-plate Kit
Precoated 96-well Strip Plate	1	2	5
Lyophilized Recombinant Standard	2	4	5
Standard Diluent	12 ml	2 x 12 ml	75 ml
Biotinylated Antibody Reagent	12 ml	2 x 12 ml	55 ml
30X Wash Buffer	50 ml	2 x 50 ml	200 ml
Streptavidin-HRP Conjugate	75 µl	2 x 75 µl	250 µl
Streptavidin-HRP Dilution Buffer	13 ml	2 x 13 ml	70 ml
Premixed TMB Substrate Solution	13 ml	2 x 13 ml	5 x 13 ml
Stop Solution	13 ml	2 x 13 ml	55 ml
Adhesive Plate Covers	6 each	12 each	30 each

 

Example standard curve for the Thermo Scientific Pierce Rat IL-6 ELISA Kit. The standard in this kit has been calibrated to a Thermo Scientific rat IL-6 internal reference standard.

Related Products:
Guide to IFN gamma Products

Rat IFN gamma (IFNg) ELISA Kit
Rat Interleukin-1 beta (IL-1 beta) ELISA Kit
Rat TNF alpha (TNFa) ELISA Kit

Instrumentation Options:
For more information on our instruments for reading ELISAs, visit www.thermo.com/readingroom.

Product #	Description	Pkg. Size
ER2IL6	Rat IL-6 Colorimetric ELISA Kit	1 plate
ER2IL62	Rat IL-6 Colorimetric ELISA Kit	2 plate
ER2IL65	Rat IL-6 Colorimetric ELISA Kit	5 plate