Maleimide Activated Plates 英文名称: Maleimide Activated Plates | ||||||||||||
型号:null 产品货号: 15150 | ||||||||||||
价格:请致电:010-57128832,18610462672 | ||||||||||||
品牌: usa | ||||||||||||
Successfully coat sulfhydryl peptides in 96-well microplates.Thermo Scientific Pierce Maleimide Activated Plates are ideal for binding sulfhydryl-containing molecules that are difficult to coat onto polystyrene plates, such as peptides that contain a terminal cysteine. These coated plates are an especially useful tool for assessing specific anti-hapten antibody titers during antibody production. Maleimide Activated Plates are available in clear for colorimetric assays, white for chemiluminescent assays, and black for fluorescent assays. Highlights:
Important Product Information Maleimides react with free sulfhydryl group(s) at pH of 6.5-7.5, forming stable thioether linkages. Reaction with amines becomes significant at pH > 7.5. Some sulfhydryl-containing peptides and proteins may oxidize in solution and form disulfide bonds, which cannot react with maleimides. Disulfide bonds can be reduced to produce free sulfhydryls. The TCEP Disulfide Reducing Gel enables peptide or protein reduction while recovering the sample in the absence of reducing agents. Sulfhydryls can be introduced via amine modification using N-succinimidyl S-acetylthioacetate (SATA) or 2-iminothiolane•HCl (Traut’s Reagent). Product # Description Pkg. Size 15150 Maleimide Activated Plates, Clear 8-well Strip Plates 5 plates 15152 Maleimide Activated White 96-Well Plates 5 plates 15153 Maleimide Activated Black 96-Well Plates 5 plates |