Proteinase K Pierce
英文名称: Proteinase K Pierce
型号:null    产品货号: 17916
价格:请致电:010-57128832,18610462672
品牌: usa

Proteinase K is a subtilisin-like endolytic protease that is isolated from the saprophytic fungus Tritirachium album. It has a high activity that is stable across a wide range of pH and temperature conditions and is suited to short digestion times. The activity of proteinase K is increased at elevated temperatures up to 65°C. Calcium is not essential to the function of proteinase K. Therefore, EDTA and other chelating agents do not interfere with the activity and may be used alongside proteinase K to inactivate calcium-dependent nucleases in DNA and RNA preparation.

Properties of Proteinase K
Alternate names Peptidase K, Tritirachium alkaline proteinase
SpecificityCleaves at the carboxyl side of aliphatic, aromatic or hydrophobic residues
Proteinase K SourceTritirachium album
Molecular weight28,900
FormLyophilized form
Concentration/activity>30 units/mg at 35°C
RNase/DNaseRNase-free and DNase-free
Protease typeSerine protease
Uses/applicationsInactivation of RNase and DNase during nucleic acid purification
Reaction conditions0.05-1 mg/ml proteinase K, pH 7.5-8, often containing 0.5-1% SDS
Storage conditionsStore at -20°C
InhibitorsPMSF or DFP

Related products
Other proteases
Protease selection chart

Product #

Description

Pkg. Size

17916

Proteinase K
Proteinase K, from tritirachium album

100 mg