Sulfhydryl- and carbohydrate-reactive crosslinker with immune response neutral spacer.

3,3´-N-[e-Maleimidocaproic acid] hydrazide, trifluoroacetic acid salt (EMCH) is a water-soluble, heterobifunctional, membrane permeable crosslinker. It contains a sulfhydryl reactive maleimide group and carbonyl reactive hydrazide group at each end of a 6-carbon spacer arm. Maleimides react with sulfhydryls at pH 6.5-7.5 to form stable thiol ether bonds, along with release of the maleimide leaving group. Proteins with cysteine residues not involved in disulfide bond formation are targets for maleimide reactive groups. Carbonyl groups, whilst not found naturally on proteins can be formed by ring sugar reduction to form aldehydes which react with hydrazides at pH5.5-7.5. EMCH Highlights Covalently couple the hydrazide group of EMCH to glycoproteins and other glycoconjugates after oxidation of sugar ring with sodium meta-periodate Sulfhydryl reactive maleimide reacts rapidly with any sulfhydryl-containing molecule Hydrazide reacts with oxidized carbohydrates M.W. 225.24, 11.8 Å spacer arm Applications for EMCH Chemical crosslinking of glycoproteins to sulfhydryl-containing molecules >Use with EDC for reactivity toward carboxyl groups Properties of EMCH Molecular formula: C10H15N3O3 Molecular weight: 225.24 Spacer arm length: 11.8 Å (6 atoms) Storage conditions: 4°C, protect from moisture, use only fresh solutions Reactive groups: Maleimide, reacts with sulfhydryls at pH 6.5-7.5 Hydrazide, reacts with carbonyl groups (oxidized carbohydrates) at pH 5.5-7.5 EMCH Specifications Pierce manufactures EMCH to the highest specifications to produce the most specific bioconjugates, ensure the integrity of your data and to provide you with the highest degree of consistency. Each lot of EMCH is tested to meet the following minimum specifications. NMR purity: >90% Melting Pt: 99-110°C Identity: IR scan shows only peaks characteristic of the structure and functional groups of EMCH Related Products KMUH         BMPH         MBPH         PDPH Bioconjugate Techniques Other popular crosslinkers   EDC (EDAC) Sulfo-SMCC Sulfo-NHS Sulfo-LC-SPDP Guide: Crosslinker Selection Guide Tech Tip: Determine reactivity of NHS ester biotinylation and crosslinking reagents

Product #	Description	Pkg. Size
22106	EMCH N-(e-Maleimidocaproic Acid) Hydrazide, trifluoroacetic acid salt	50 mg