Immunodiffusion Plates
英文名称: Immunodiffusion Plates
型号:null    产品货号: 31111
价格:请致电:010-57128832,18610462672
品牌: usa

Pre-cast Immunodiffusion plates provide antibody-antigen precipitation detection.

Immunodiffusion Plates

Immunodiffusion (ID) is a classic technique for detecting antibody-antigen interactions based on the theory of double diffusion originally described by Oudin 1 and Ouchterlony. 2 Antigens and antibodies are placed into separate wells that are cut into a gel matrix and allowed to diffuse towards each other. If the reaction is positive, a precipitate forms that appears as an opaque line. The precipitation reaction occurs when the antigen and antibody concentrations are combined at near equivalent proportions.

When multivalent antigens combine with divalent antibodies in solution, three-dimensional lattices are formed that aggregate and precipitate. The amount of precipitate varies in proportion to the concentration of the antigen and antibody. At equivalent or optimal proportions almost all the antigen and antibody will precipitate. If there is an excess of antibody, the complexes formed with the antigen are insoluble. When there is an excess of antigen, the precipitate has a tendency to dissolve caused by the formation of soluble complexes.

Advantages

  • Gelling agent contains precipitin brighteners for easy visualization
  • Immunodiffusion plates contain diffusion enhancers to help speed the interaction process
  • Excellent precipitin bands with antibodies from all species (including rabbit antibodies)
  • Gels can be washed, dried and stained for a permanent record
 Single pattern platesMultiple pattern plates
Well Diameter5.0 mm3.2 mm
Interwell Distance4 mm 3.2 mm
Gel Depth1.6 mm2.8 mm
Well Volume31 μl22 μl

Product #

Description

Pkg. Size

31111

Immunodiffusion Plates, Agarose Gelling Agent, Multiple Pattern

6 PLATES

31113

Immunodiffusion Plates, Agarose Gelling Agent, Single Pattern

10 PLATES