英文名称： Slide-A-Lyzer Dialysis Cassettes, 10K MWCO
Faster and easier to use than dialysis tubing!
Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) facilitate simple and effective removal of buffer salts and small contaminants from proteins and other macromolecules that are larger than 10,000 daltons.
Available in four different cassette sizes to accommodate sample volumes between 0.1 and 30mL, these devices are easier to use than traditional dialysis tubing, providing both faster buffer exchange and more complete sample recovery. Slide-A-Lyzer Cassettes can be used for a wide range of applications including low molecular-weight contaminant removal, buffer exchange, desalting, equilibrium dialysis, and sample concentration.
The cassettes are constructed from two sheets of low-binding, regenerated-cellulose dialysis membrane that are hermetically sealed on either side of a silicone-like gasket inside an inert plastic frame. The membrane and cassette materials are compatible with most common laboratory chemicals and buffers. Liquid samples are easily added and removed by penetrating the self-sealing gasket with a hypodermic needle attached to a syringe. No knots, clips or caps are needed to seal the units and prevent leaking and sample-loss during the dialysis procedure.
Gamma-irradiated (Sterile) Dialysis Cassettes:
Gamma-irradiated Slide-A-Lyzer Dialysis Cassettes are ideal for researchers culturing cells and microorganisms; purifying viruses, DNA and RNA; or performing sample preparation for other applications in which sterile conditions are required to minimize the risk of sample contamination. The gamma-irradiated cassettes are treated with 20-36 kGy of gamma radiation, a dosage that has been determined to sterilize the units based on both trypticase soy broth medium for identifying total growth and fluid thioglycollate medium for identifying anaerobic growth.
Slide-A-Lyzer Dialysis Cassette Procedure: | ||||
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| 1. Insert syringe needle through the gasket via one of the corner ports of the cassette. Inject the sample, withdraw the excess air and remove the syringe. | 2. Attach a float buoy and dialyze. (Buoys also serve as convenient bench-top stands for the cassettes.) | 3. Insert empty syringe needle at a second corner port. Inject air to expand the cassette chamber, then withdraw the dialyzed sample. | ||
Highlights:
- Easy to use – no knots or clamps are needed; just inject sample into cassette and begin dialysis
- Fast dialysis – flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing
- High recovery – rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports
- Four cassette sizes – select the cassette that best suits your sample volume
- Color-coded – orange cassette frame specifies cassettes with the 10,000 MW pore size membrane
10K Dialysis Membrane Specifications:
- Composition: Regenerated cellulose synthesized by the Viscose method
- Thickness: 0.9 to 1.2mil (22.5 to 30µm)
- Hydration Required Before Use: 30 seconds for small-volume samples
- Glycerol Content: 21%
- Sulfur Content: 0.05%
- Heavy Metals Content: Trace
Additional Information:
 | Characterization of 10K membrane performance. Dialysis membrane for Thermo Scientific Slide-A-Lyzer Dialysis Cassettes (10K MWCO) were evaluated with the following protein samples: insulin chain B (3.5 kDa), insulin (5 kDa), and cytochrome C (12.5 kDa). The samples (1mg/mL) in either 0.15M sodium chloride or 0.2M carbonate-bicarbonate buffer pH 9.4 were dialyzed overnight (16 hours) at room temperature in a rotating cell. The retentate concentration of protein was estimated by absorbance at 280nm. Results indicate that the effective membrane pore size is approximately 10K. |
Product # | Description | Pkg. Size |
66383 | Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 0.5ml | 10 cassettes |
66384 | Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 0.5ml | 5 x 10 cassettes |
66385 | Slide-A-Lyzer Dialysis Cassette Kit, 10K MWCO, 0.5ml | Kit |
66380 | Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 3ml | 10 cassettes |
66381 | Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 3ml | 5 x 10 cassettes |
66382 | Slide-A-Lyzer Dialysis Cassette Kit, 10K MWCO, 3ml | Kit |
66405 | Slide-A-Lyzer Dialysis Cassette Trial Kit, 10K MWCO, 3ml | Kit |
66810 | Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 12ml | 8 cassettes |
66811 | Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 12ml | 5 x 8 cassettes |
66807 | Slide-A-Lyzer Dialysis Cassette Kit, 10K MWCO, 12ml | Kit |
66830 | Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 30ml | 6 cassettes |
66454 | Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 0.5ml, gamma-irradiated | 10 cassettes |
66455 | Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 3ml, gamma-irradiated | 10 cassettes |
66453 | Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 12ml, gamma-irradiated | 8 cassettes |
66456 | Slide-A-Lyzer Dialysis Cassettes, 10K MWCO, 30ml, gamma-irradiated | 6 cassettes |