英文名称： Pierce C18 Tips
Monolithic C18 sorbent in a pipette tip for fast sample desalting and concentrating.
The Thermo Scientific Pierce C18 Tips enable fast and efficient capture, concentration, desalting and elution of peptides and proteins. The five-step procedure is simple and requires less than 5 minutes to process protein digests, strong cation exchange fractions and other protein and peptide samples for mass spectrometric (MS) analyses. The Pierce C18 Tips offer excellent flow properties with a high-efficiency monolithic C18 sorbent for fast wetting, loading, washing and eluting.
Improve protein analysis results with Pierce C18 Tips by removing urea, salts and other contaminants before MS analysis. The tips are ideal for matrix-assisted laser desorption ionization (MALDI) or nanoelectrospray ionization techniques. The Pierce C18 Tips are available in convenient 10 and 100µl tips with binding capacities of 8 and 80µg, respectively.
Highlights of Pierce C18 Tips:
- Monolithic C18 fast-flow tips have low resistance and improved flow characteristics compared to other commercially available tips
- High-binding capacity in a convenient tip format
- Two pipette tip formats for processing up to 8 or 80µg of sample
- Eight packs and 96-tip trays are automation compatible
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| Removing urea and NaCl eliminates interference in MS chromatograms. A bovine serum albumin (BSA) tryptic digest was analyzed on a Thermo Scientific MALDI-Orbitrap XL Hybrid Mass Spectrometer. Digests and samples containing 150mM NaCl and 4M urea were analyzed with or without processing with Pierce C18 Tips (10µl). |
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| The Thermo Scientific Pierce C18 Tips outperform other suppliers tips. BSA tryptic digests were analyzed either directly on a Thermo Scientific LTQ-XL Ion Trap Mass Spectrometer or after processing with Pierce C18 Tips (100µl) or supplier X tips. Base peak chromatograms of the peptide elution were extracted from the data sets to evaluate sample complexity and chromatographic resolution. MS results were analyzed with Matrix Science Mascot and the SwissProt Release 52 database to determine protein sequence coverage. |
Related Products:
Mass Spectrometry-Grade Endoproteinases Product # Description Pkg. Size 87781 Pierce C18 Tips, 10µl bed 8 tips 87782 Pierce C18 Tips, 10µl bed 96 tips 87783 Pierce C18 Tips, 100µl bed 8 tips 87784 Pierce C18 Tips, 100µl bed 96 tips
Pierce C-18 Spin Columns
AQUA and Heavy Peptides
for up to 8µg of sample
for up to 8µg of sample
for up to 80µg of sample
for up to 80µg of sample