A complete, optimized ELISA kit specific for porcine TNF alpha (TNFa).

EP2TNFA a complete ELISA kit for porcine TNF alpha. The Thermo Scientific Pierce Porcine TNF alpha ELISA Kit is a colorimetric enzyme-linked immunosorbent assay for quantification of porcine tumor necrosis factor alpha in serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples.

The kit features a user-friendly procedure, short assay time, and small sample size – ideal for researchers working with 96-well cell culture experiments.

The assay standard is calibrated to an international reference standard and each kit lot is carefully tested to ensure optimal performance and consistency from one lot to the next. Culture supernatant, serum, and plasma samples can be run together in the same assay using a single standard curve.

Porcine TNF alpha ELISA Kit specifications:

Assay Range: 31.3-2,000 pg/ml
Sensitivity: less than 5 pg/ml
Assay Time: 4 hours
Sample Volume: 50 µl per well
Format: 96-well strip plate
Detection: colorimetric (TMB substrate)
Validated Sample Types: culture supernatant, serum and plasma (EDTA, sodium citrate and heparin)
Specificity: This ELISA uses antibodies raised against human TNF alpha. This ELISA reacts with porcine and human TNF alpha at 100%. The following cytokines, tested at 1 µg/ml, did not interfere or cross-react: human IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12p70, IL-12p40, IL-13, IL-15, IL-16, IL-17, IL-18, Eotaxin, GCSF, GMCSF, GRO alpha, IFN alpha, IFN gamma, MCP-1, MCP-2, MCP-3, MCP-4, VEGF, MIP-1 alpha, MIP-1 beta, TGF beta, TNF alpha or RANTES; mouse TNF alpha; rat TNF alpha; bovine TNF alpha.

Porcine TNF alpha ELISA protocol. Click on image to obtain complete product instructions including a graphical procedure summary and descriptions of sample preparation and dilution of standards.

Kit Components	Quantity
Precoated 96-well Strip Plate	1
Lyophilized Recombinant Standard	2 vials
Standard Diluent	14 ml
Biotinylated Antibody Reagent	12 ml
30X Wash Buffer	50 ml
Streptavidin-HRP Reagent	14 ml
Premixed TMB Substrate Solution	13 ml
Stop Solution	13 ml
Adhesive Plate Covers	6 each

Standard curve for Pierce Porcine TNF alpha ELISA Kit.

Example standard curve for the Thermo Scientific Pierce Porcine TNFa ELISA Kit. The standard in this kit has been calibrated to an in-house porcine TNF alpha standard.

Instrumentation Options:
For more information on our instruments for reading ELISAs, visit www.thermo.com/readingroom.
Product #
Description
Pkg. Size
EP2TNFA
Porcine TNF alpha Colorimetric ELISA Kit
1 plate