英文名称： Pierce Protein Gels
Precast gels with long shelf life and reproducible migration patterns.
Thermo Scientific Pierce Protein Gels have long shelf life and assured reproducibility of protein migration pattern. Used with the Tris-HEPES running buffer, these durable precast Pierce Protein Gels provide fast separation with resolution that is similar to traditional Laemmli PAGE gel systems.
Highlights of the Pierce Precast Gels:
- Highly visible sample wells
- 45 minute average run time
- Easy-open cassettes have no combs or tape
- 10 times more durable than conventional homemade or commercial gels
- Reinforced sample wells eliminate damage to wells while loading, preventing well-to-well contamination
- Tris-HEPES running buffer system for improved electrophoretic performance and long shelf life
About the Pierce Protein Gels
The Tris-glycine Laemmli system is the most popular buffer formulation for SDS-PAGE. This system, however, is limited by extended run times, fragility and short shelf life caused by the hydrolysis of polyacrylamide to acrylic acid in alkaline conditions. This chemical change alters the conductivity of the gel that adversely affect the consistency of protein migration. Pierce Protein Gels overcome these problems. They are more rigid and ten times more durable than conventional pre-cast gels. Pierce Protein Gels are cast using a method that yields more uniform and complete polymerization of the acrylamide than traditional, chemical-based methods. As a result, Pierce Protein Gels have superior batch-to-batch reproducibility and contain no unpolymerized acrylamide.
| Pierce Protein Gel Specifications | |
| Cassette Dimensions: | 10cm x 10cm x 7mm |
| Gel Dimensions: | 8cm x 8.5cm x 1mm |
| Storage Conditions: | 1 year at 4°C |
| Stacking Gel: | 4% polyacrylamide |
| Buffer System in Gel: | pH 8.5 |
| SDS Content: | 1% |
| Required Running Buffer: | Tris-HEPES-SDS |
| Recommended Sample Buffer: | Tris-HCl-LDS (Lithium Dodecyl Sulfate) |
| Pierce Protein Gels are compatible with the Thermo Scientific Owl P82 System, Life Technologies XCell II* and Surelock* Systems, CBS Scientific CBDCX-700 Dual Cool and CBEBX-700 4-place Blotting Systems, Inova Biosciences PAGEr* Minigel Chamber and other gel tanks and apparatus that accommodate cassette dimensions of 10cm x 10cm x 4cm. | |
 | 4-20% Pierce Protein Gel stained with GelCode Blue Stain. Proteins were separated on a 4-20% 17-well Pierce Protein Gel, washed 30 minutes with water, stained for 60 minutes with GelCode Blue Stain and destained 3 x 20 minutes with water. Lane 1-2, 10, 17: SDS-PAGE Molecular Weight Standards, Broad Range (Bio-Rad) (5µL) Lane 3-4: Hela Cell Lysate (1.88µg) Lane 5-6: Purified BSA (0.3µg) Lane 7-8: E. coli lysate (1.88µg) Lane 9: Precision Plus Protein Standards Dual Color (Bio-Rad) (5µL) Lane 11-12: HeLa cell lysate (0.88µg) Lane 13-14: Purified BSA (0.15µg) Lane 15-16: E. coli lysate (0.88µg) |
 4-8% Pierce Protein Gel |  12% Pierce Protein Gel |
| 4-8% and 12% Pierce Protein Gel stained with GelCode Blue Stain. Proteins were separated on a 4-8% or 12% 12-well Pierce Protein Gel, washed 3 x 10 minutes with water, stained for 60 minutes with GelCode Blue Stain and destained 3 x 20 minutes with water. Lane 1-2: SDS-PAGE Molecular Weight Standards, Broad Range (Bio-Rad) (5µL) Lane 3-4: Bovine Serum Albumin (0.3µg) Lane 5-6: Blue Carrier Hemocyanin protein (0.3µg) Lane 7-8: Jurkat cell lysate (1.88µg) Lane 9-10: A549 cell lysate (1.88µg) Lane 11-12: MOPC cell lysate (1.88µg) | |
| Chemiluminescent and Fluorescent Western blot detection of Cytokeratin 18. | |
| Chemiluminescent detection. A549 cell lysate was separated on a 10-20% 12-well Pierce Protein Gel and transferred to nitrocellulose. The blot was blocked overnight with 1% BSA, probed for 60 minutes with rabbit anti-cytokeratin 18 antibody and detected with a HRP-conjugated goat anti-rabbit secondary antibody. The blot was incubated for 5 minutes in Pierce ECL Western Blotting Substrate and exposed to CL-Xposure film for 1 minute. Lane 1-5 contained A549 cell lysate at 10µg, 5µg, 2.5µg, 1.25µg and 0.625µg, respectively. Lane 6: MW marker. |  |
| Fluorescent detection. HeLa cell lysate was separated on a 12% 12-well Pierce Protein Gel and transferred to Low Fluorescence PVDF. The blot was blocked for 60 minutes in SEA BLOCK Protein Blocker, probed for 60 minutes with rabbit anti-cytokeratin 18 antibody and detected with a DyLight 680B-conjugated goat anti-rabbit secondary antibody. The image was captured on Licor Odyssey* Instrument at 700 Channel. Lane 1-5 contained HeLa cell lysate at 5µg, 2.5µg, 1.25µg, 0.625µg and 0.312µg, respectively. Lane 6: MW marker. |  |
Related Products and Resources:
LDS Sample Buffer (4X) – sample loading buffer recommended for use with Pierce Precast Gels Product # Description Pkg. Size 84708 Pierce Protein Gels, 4-8%, 12 Well 10 gels 84710 Pierce Protein Gels, 4-8%, 17 Well 10 gels 84711 Pierce Protein Gels, 12%, 12 Well 10 gels 84712 Pierce Protein Gels, 12%, 17 Well 10 gels 84713 Pierce Protein Gels, 4-20%, 12 Well 10 gels 84714 Pierce Protein Gels, 4-20%, 17 Well 10 gels
BupH Dry Blend Electrophoresis Buffers – Tris-HEPES-SDS and other buffers in conventient pouches
Protein Molecular Weight Markers – product comparison and selection guide
Protein Gels Stains and Kits – compare colorimetric and fluorescent gel stains
Secondary Antibodies and Conjugates – compare secondary detection reagents and conjugates
Western blotting substrates and reagents – find kits, stripping buffers, blotting membranes and film