Pierce Chromatography Cartridges
英文名称： Pierce Chromatography Cartridges
型号:null    产品货号： 89971
价格:请致电：010-57128832,18610462672
品牌: usa

Affinity and desalting resins in cartridges for liquid chromatography (LC).

Pierce Chromatography CartridgesThe Thermo Scientific Pierce Chromatography Cartridges are convenient, reliable, ready-to-use, pre-packed 1mL and 5mL columns of sample-prep and affinity-purification resins for manual or automated liquid chromatography (LC).

The cartridge-fittings are compatible with the popular automated liquid-chromatography systems or for manual syringe processing. The cartridges attach directly to ÄKTA* or FPLC Systems without additional connectors. Cartridges can be used individually or connected in a series to obtain even higher column capacity. Each product supplied in the Pierce Chromatography Cartridge format includes an accessory pack that readily adapts cartridges for use with Luer-Lok* Syringe Fittings or tubing. The cartridges provide fast, easy and reproducible chromatographic separations and can be regenerated for multiple uses.

Chromatography Cartridge Highlights:

  • Two sizes – 1mL and 5mL, convenient for typical research scales
  • Compatible – fittings allow connection with popular LC systems or a standard syringe
  • Versatile – use singly or connected in series to service different capacity requirements
  • Validated – available cartridges have been tested to ensure performance in the format
  • Reusable – accessory pack includes caps for convenient storage between uses
  • Economical – comparable performance at lower cost than other commercially available cartridges
Pierce Chromatography Cartridge properties.
  1 ml Cartridge5 ml Cartridge
Dimensions0.7 x 2.7cm1.3 x 3.8cm
Recommended Flow Rate1-4mL/minute1-7mL/minute
Maximum Pressure0.3 mPa (43 psi or 3 bar)0.3 mPa (43 psi or 3 bar)
Cartridge Materialpolypropylenepolypropylene
Frit Materialpolyethylenepolyethylene

Performance Data:

Sample desalting performance of Zeba Desalting Matrix in Pierce Chromatography Cartridges.
Efficient salt removal and protein recovery with Desalting Chromatography Cartridge. Bovine serum albumin (1mg) in 1M NaCl was applied to 5mL Thermo Scientific Zeba Desalting Cartridge at a flow rate of 5mL/minute. Cartridge profile shows isocratic elution of BSA (blue) and NaCl detected by conductivity (brown). Greater than 95% of the BSA was recovered and more than 95% of the was salt removed. Results were comparable to those obtained with desalting cartridges from other suppliers (see Note).

 

Antibody purification performance of Pierce Protein A Agarose Resin in Pierce Chromatography Cartridges.
Excellent antibody yield and purity acheived with Protein A Chromatography Cartridge. Normal human serum (60mg) was applied in PBS to a 1mL Thermo Scientific Pierce Protein A Cartridge and eluted with 0.1M glycine, pH 2.8, using a flow rate of 1mL/minute. The arrow denotes the start of the low pH elution. The yield of human IgG was 6.85mg. Fractions were separated by SDS-PAGE and the gel was stained with Imperial* Protein Stain (Product # 24615). M = MW marker, L = sample load, FT = flow-through and E = elution. Results were comparable to those obtained with a GE Healthcare HiTrap* Column (see Note).

Note: see additional Performance Data for Pierce Chromatography Cartridges, including side-by-side comparison to more expensive columns from other suppliers.

Available Pierce Chromatography Cartridge Products: