Affinity and desalting resins in cartridges for liquid chromatography (LC).
The Thermo Scientific Pierce Chromatography Cartridges are convenient, reliable, ready-to-use, pre-packed 1mL and 5mL columns of sample-prep and affinity-purification resins for manual or automated liquid chromatography (LC).
The cartridge-fittings are compatible with the popular automated liquid-chromatography systems or for manual syringe processing. The cartridges attach directly to ÄKTA* or FPLC Systems without additional connectors. Cartridges can be used individually or connected in a series to obtain even higher column capacity. Each product supplied in the Pierce Chromatography Cartridge format includes an accessory pack that readily adapts cartridges for use with Luer-Lok* Syringe Fittings or tubing. The cartridges provide fast, easy and reproducible chromatographic separations and can be regenerated for multiple uses.
Chromatography Cartridge Highlights:
- Two sizes – 1mL and 5mL, convenient for typical research scales
- Compatible – fittings allow connection with popular LC systems or a standard syringe
- Versatile – use singly or connected in series to service different capacity requirements
- Validated – available cartridges have been tested to ensure performance in the format
- Reusable – accessory pack includes caps for convenient storage between uses
- Economical – comparable performance at lower cost than other commercially available cartridges
Pierce Chromatography Cartridge properties. |
| 1 ml Cartridge | 5 ml Cartridge |
Dimensions | 0.7 x 2.7cm | 1.3 x 3.8cm |
Recommended Flow Rate | 1-4mL/minute | 1-7mL/minute |
Maximum Pressure | 0.3 mPa (43 psi or 3 bar) | 0.3 mPa (43 psi or 3 bar) |
Cartridge Material | polypropylene | polypropylene |
Frit Material | polyethylene | polyethylene |
Performance Data:
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Efficient salt removal and protein recovery with Desalting Chromatography Cartridge. Bovine serum albumin (1mg) in 1M NaCl was applied to 5mL Thermo Scientific Zeba Desalting Cartridge at a flow rate of 5mL/minute. Cartridge profile shows isocratic elution of BSA (blue) and NaCl detected by conductivity (brown). Greater than 95% of the BSA was recovered and more than 95% of the was salt removed. Results were comparable to those obtained with desalting cartridges from other suppliers (see Note). |
Recombinant Mouse IFN alpha A
1x105 |