Protino® Ni-TED Resin	Purification of His-tag proteins
Features
Protino® Ni-TED, the pure alternative for His-tag protein purification
• Chelating group TED (tris(carboxymethyl)ethylene diamine), one protein binding     site provides highest selectivity and purity (also see Technologies) • High stability against reducing / chelating agents • Low metal leaching • High protein purity • High binding specificity, less unspecific binding of contaminating proteins     compared to other common IMAC matrices • Dry resin – storage at room temperature Protino® Ni-TED Resin • Dry bulk material • Rigid support silica matrix, compatible with high pressure and high flow rates,     does not shrink or swell – highest flexibility • Batch procedure, gravity-flow column chromatography, FPLC™ applications • Empty Protino® columns available for use with Protino® Ni-TED Resin • Empty 96-well Receiver Plates available for use with Protino® Ni-TED Resin

Technology	IMAC (immobilized metal ion affinity chromatography)
Chelating ligand	TED (tris-carboxymethyl ethylene diamine)
Format	Bulk matrix
Matrix	Macroporous silica
Physical form	Dry matrix, precharged with Ni2+
Binding capacity*	10 mg/g resin 5 mg/mL bed volume
Max. pressure	145 psi (10 bar)
* Binding capacity will vary for each polyhistidin-tagged protein
Applications
• Purification of polyhistidine-tagged proteins • Batch binding, gravity-flow column chromatography, MPLC / FPLC™ applications

Ordering information

Product	Preps	Specification	REF
Protino® Ni-TED Resin	5 g	dry silica bulk resin	745200.5
	30 g	as above	745200.30
	120 g	as above	745200.120
	600 g	as above	745200.600
Product accessories	Pack of	Specification	REF
Protino® Columns 14 mL	10	empty columns (gravity flow) for use with e.g. Protino® Ni-TED / IDA Resin	745250.10
Protino® Columns 35 mL	10	empty columns (gravity flow) for use with e.g. Protino® Ni-TED / IDA Resin	745255.10
Receiver Plate 50 µm hydrophilized	4	96-well plates with an inserted hydrophilized filter frit of 50 µm pore size, suitable for gravity flow, centrifugation, and vacuum	740689.4