| 人VEGF-CELISA试剂盒 英文名称： Human VEGF-C ELISA Kit | |
| 型号:null 产品货号： EK0588 | |
| 价格:请致电：010-57128832,18610462672 | |
| 品牌: 美国 | |
产品：人VEGF-CELISA试剂盒 货号：EK0588 Description Vascular endothelial growthFactor C (VEGF-C) is a member of the platelet-derived growthFactor/vascular endothelial growthFactor (PDGF/VEGF)Family. The human gene encoding it is VEGFC, and is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processedForms which bind and activate VEGFR-3 receptors. Only theFully processedForm can bind and activate VEGFR-2 receptors. This protein is structurally andFunctionally similar to vascular endothelial growthFactor D (VEGF-D). The C terminus of VEGF-C has cysteine-rich repeat units characteristic of the Balbiani ring 3 protein (BR3P) of the midge Chironomus tentans. The standard product used in this kit is recombinant human VEGF-C, consisting of 135 amino acids with a molecular mass of 23kDa after glycosylation. ScienCells human VEGF-C ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human VEGF-C-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human VEGF-C in the sample that is captured on the strips. Product Use This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. Storage Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles. Shipping Shipped on gel ice Reference 1. Joukov, V.; Pajusola, K.; Kaipainen, A.; Chilov, D.; Lantinen, I.; Kukk, E.; Saksela, O.; Kalkkinen, N.; Alitalo, K. : A novel vascular endothelial growthFactor, VEGF-C, is a ligandFor theFlt4 (VEGFR-3) and KDR (VEGFR-2) receptor tyrosine kinases. EMBO J. 15: 290-298, 1996. 2. Lee, J.; Gray, A.; Yuan, J.; Luoh, S.-M.; Avraham, H.; Wood, W. I. : Vascular endothelial growthFactor-related protein: a ligan
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