人过氧化物酶ELISA试剂盒 英文名称: Human Myeloperoxidase (MPO) ELISA Kit | |
型号:null 产品货号: EK0850 | |
价格:请致电:010-57128832,18610462672 | |
品牌: 美国 | |
产品:人过氧化物酶ELISA试剂盒 货号:EK0850 Description Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsibleFor microbicidal activity against a wide of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resultingFrom oxygen radicals produced during myeloid cell maturation andFunction. The standard product used in this kit is recombinant MPO, consisting of 697 (A49-S745) amino acids with a molecular mass of 80 kDa. ScienCells human MPO ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human MPO-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human MPO in the sample that is captured on the strips. Product Use This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. Storage Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles. Shipping Shipped on gel ice Reference 1. Klebanoff, S. J. : Myeloperoxidase. Proc. Assoc. Am. Phys. 111: 383-389, 1999. 2. Murao, S.-I.; Stevens,F. J.; Ito, A.; Huberman, E. : Myeloperoxidase: a myeloid cell nuclear antigen with DNA-binding properties. Proc. Nat. Acad. Sci. 85: 1232-1236, 1988. 3. Nauseef, W. M.; Olsson, I.; Arnljots, K. : Biosynthesis and processing of myeloperoxidase--a markerFor myeloid cell differentiation. Europ. J. Haemat. 40: 97-110, 1988. 如需要其他产品资料请发邮件至wwwfudan@163.com索取
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