Description

Interleukin-27 (IL-27) is a heterodimeric cytokine belonging to the IL-12Family that is composed of two subunits: Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (also known as IL-30). IL-27 is produced by antigen-presenting cells and plays an important role in regulating the activity of B- and T-lymphocytes. IL-27, a potent inhibitor of T(H)-17 cell development, may be a useful targetFor treating inflammatory diseases mediated by these cells.

ScienCells human IL-27 ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human IL-27-specific monoclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human IL-27 in the sample that is captured on the strips.

Product Use

This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.

Storage

Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.

Shipping

Shipped on gel ice

Reference

1. Larousserie,F; Bardel, E; Pflanz, S; Arnulf, B; Lome-Maldonado, C; Hermine, O; Bregeaud, L; Perennec, M et al (April 2005). "Analysis of interleukin-27 (EBI3/p28) expression in Epstein-Barr virus- and human T-cell leukemia virus type 1-associated lymphomas: heterogeneous expression of EBI3 subunit by tumoral cells". The American Journal of Pathology 166 (4): 1217-28.
2. Lucas S, Ghilardi N, Li J, de SauvageFJ (December 2003). "IL-27 regulates IL-12 responsiveness of naive CD4+ T cells through Stat1-dependent and -independent mechanisms". Proceedings of the National Academy of Sciences of the United States of America 100 (25): 15047-52.
3. LarousserieF, Charlot P, Bardel E,Froger J, Kastelein RA, Devergne O (May 2006). "Differential effects of IL-27 on human B cell subsets". Journal of Immunology (Baltimore, Md. : 1950) 176 (10): 5890-7.
4. Stumhofer, J. S., Laurence, A., Wilson, E. H., Huang, E., Tato, C. M., Johnson, L. M., Villarino, A. V., Huang, Q., Yoshimura, A., Sehy, D., Saris, C. J. M., OShea, J. J., Hennighausen, L., Ernst, M., Hunter, C. A. Interleukin 27 negatively regulates the development of interleukin 17-producing T helper cells during chronic inflammation of the central nervous system. Nature Immun. 7: 937-945, 2006.