| 人PAI-1ELISA试剂盒 英文名称： Human PAI-1 ELISA Kit | |
| 型号:null 产品货号： EK0859 | |
| 价格:请致电：010-57128832,18610462672 | |
| 品牌: 美国 | |
产品：人PAI-1ELISA试剂盒 货号：EK0859 Description Plasminogen activator inhibitor-1 (PAI-1) is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and henceFibrinolysis (the physiological breakdown of blood clots). PAI-1 is a serine protease inhibitor (serpin) protein (SERPINE1). PAI-1 is mainly produced by the endothelium (cells lining blood vessels), but is also secreted by other tissue types, such as adipose tissue. The PAI-1 gene is located on chromosome 7 (7q21.3-q22). Congenital deficiency of PAI-1 leads to a hemorrhagic diathesis (a tendency to hemorrhage). PAI-1 is present in increased levels in various disease states (such as a number ofForms of cancer), as well as in obesity and the metabolic syndrome. It has been linked to the increased occurrence of thrombosis in patients with these conditions. Additionally, it has been shown to interact with ORM1. The standard product used in this kit is recombinant human PAI-1 with a molecular mass of 43 kDa. ScienCells human PAI-1 ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human PAI-1-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human PAI-1 in the sample that is captured on the strips. Product Use This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. Storage Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles. Shipping Shipped on gel ice Reference 1. Klinger, K. W.; Winqvist, R.; Andreasen, P. A.; Stuart, N.; Stanislovitis, P.; Schwartz, R.; Doherty, R.; Alitalo, K.; Blasi,F.; Dano, K. : Regional mapping of the plasminogen activator inhibitor type 1 gene to 7q21.3-q22 and assignment to the CF linkage group. (Abstract) Cytogenet. Cell Genet. 46: 640 only, 1987. 2. Boncela, J; Papiewska I,Fijalkowska I, Walkowiak B, Cierniewski C S (Sep. 2001). "Acute phase protein alpha 1-acid glycoprotein interacts with plasminogen activator inhibitor type 1 and stabilizes its inhibitory activity". J. Biol. Chem. (United States) 276 (38): 35305-11. 如需要其他产品资料请发邮件至wwwfudan@163.com索取
|