人 IL-5酶联免疫试剂盒 英文名称: Human IL-5 ELISA Kit | |
型号:null 产品货号: EK0407 | |
价格:请致电:010-57128832,18610462672 | |
品牌: 美国 | |
产品:人 IL-5酶联免疫试剂盒 货号:EK0407 Description Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31.3 Human and mouse of IL-5 share 70% amino acid sequence homology. ScienCells human IL-5 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human IL-5-specific monoclonal antibodies are precoated onto 8 x 12 strips. The human specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human IL-5 in the sample that is captured in the strips.
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.
Shipped on gel ice.
1. Pereira, E.; Goldblatt, J.; Rye, P.; Sanderson, C.; Le Souef, P. Mutation analysis of interleukin-5 in an asthmatic cohort. Hum. Mutat. 11: 51-54, 1998.
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