| 人IGFBP-3酶联免疫试剂盒 英文名称： Human IGFBP-3 ELISA Kit |
| 型号:null 产品货号： EK0386 |
| 价格:请致电：010-57128832,18610462672 |
| 品牌: 美国 |
上海复蒙基因生物科技有限公司是美国Sciencell公司在中国正规一级代理： 美国ScienCell研究实验室（ www.sciencellonline.com ）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在全球拥有众多客户。在国内销售8年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严格的质控和优秀的产品品质，深受广大科研工作者的信赖。 ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。 确保了实验结果的重复性和连贯性。 请登录Sciencell公司官方网站（ www.sciencellonline.com 或 www.sciencell-sh.com ）以确保购买正规公司产品。 产品：人IGFBP-3酶联免疫试剂盒 货号：EK0386 Description ***-like growth factor-binding protein-3 (IGFBP-3) is a major determinant of circulating levels of the IGFs and is clinically useful for the evaluation of GH deficiency and for predicting the response to GH treatment. The circulating level of IGFBP-3 is inversely related to the risk of several common cancers, and antiproliferative agents such as antiestrogens and retinoids act in part by up-regulating IGFBP-3 expression. IGFBP-3, well-characterized as the carrier of IGF, has been reported to have intrinsic bioactivity that is independent of IGF binding; IGFBP-3 has an IGF-independent, antiproliferative effect in undifferentiated and early differentiated, but not in terminally differentiated, chondrocytes. IGFBP-3 possesses both growth-inhibitory and potentiating effects on cells that are independent of IGF action and are mediated through specific IGFBP-3 binding proteins/receptors locate at the cell membrane, cytosol, or nuclear compartments and in the extra cellular matrix. IGFBP-3 is located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-3, consisting of 265 amino acids with the molecular mass of 29kDa. As a result of glycosylation, the molecular mass is 41KDa. ScienCells human IGFBP-3 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human IGFBP-3 specific-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Bioyin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human IGFBP-3 captured in strips .
This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.
Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.
Shipped on gel ice.
1. Deal, C.; Ma, J.; Wilkin, F.; Paquette, J.; Rozen, F.; Ge, B.; Hudson, T.; Stampfer, M.; Pollak, M. Novel promoter polymorphism in insulin-like growth factor-binding protein-3: correlation with serum levels and interaction with known regulators. J. Clin. Endocr. Metab. 86: 1274-1280, 2001. 如需要其他产品资料请发邮件至wwwfudan@163.com索取 |