人uPARElisa试剂盒 英文名称: Human uPAR ELISA Kit | |
型号:null 产品货号: EK0536 | |
价格:请致电:010-57128832,18610462672 | |
品牌: 美国 | |
产品:人uPARElisa试剂盒 货号:EK0536 Description The urokinase-type plasminogen activator receptor (uPAR) is a key molecule in the regulation of cell-surface plasminogen activation and as such, plays an important role in many normal and pathological processes. Mo3 is an activation antigen expressed by human monocytes and myelomonocytic cell lines after stimulation by a variety of agents. Mo3 expression in vivo is associated predominantly with macrophages at inflammatory sites. It is a highly glycosylated protein of about 50kDa, where it is anchored to the plasma membrane by glycosyl-phosphatidylinositol linkage. The complete coding sequence of the cDNA has beenFound to encode 335 amino acids, including a predicted signal peptide of 22 residues and a hydrophobic C-terminal portion. Mo3 is identical to the human receptorFor the urokinase-type plasminogen activator (uPA). uPAR is a useful prognostic markerFor biologically aggressiveForms of endometrial cancer. uPAR is located at chromosome 19q13.1-q13.2. The standard product used in this kit is recombinant human uPAR, consisting of 287 amino acids with a molecular mass of 31kDa. ScienCells human uPAR ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human uPAR-specific polyclonal antibodies are pre-coated onto 8 x 12 strips. The human-specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human uPAR in the sample that is captured on the strips. Product Use This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. Storage Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles. Shipping Shipped on gel ice Reference 1. Borglum, A. D.; Byskov, A.; Ragno, P.; Roldan, A. L.; Tripputi, P.; Cassani, G.; Dano, K.; Blasi,F.; Bolund, L.; Kruse, T. A. Assignment of the urokinase-type plasminogen activator receptor gene (PLAUR) to chromosome 19q13.1-q13.2. Am. J. Hum. Genet. 50: 492-497, 1992. 2. Min, H. Y.; Semnani, R.; Mizukami, I.F.; Watt, K.; Todd, R.F., III; Liu, D. Y. cDNAFor Mo3, a monocyte activation antigen, encodes the human receptorFor urokinase plasminogen activator. J. Immun. 148: 3636-3642, 1992. 3. Memarzadeh, S.; Kozak, K. R.; Chang, L.; Natarajan, S.; Shintaku, P.; Reddy, S. T.;Farias-Eisner, R. Urokinase plasminogen activator receptor: prognostic biomarkerFor endometrial cancer. Proc. Nat. Acad. Sci. 99: 10647-10652, 2002. Note: Erratum: Proc. Nat. Acad. Sci. 99: 12501 only, 2002.
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