| 人p53 ELISA试剂盒 英文名称： Human p53 ELISA Kit | |
| 型号:null 产品货号： EK0895 | |
| 价格:请致电：010-57128832,18610462672 | |
| 品牌: 美国 | |
产品：人p53 ELISA试剂盒 货号：EK0895 Description p53 (also known as protein 53 or tumor protein 53), is a tumor suppressor protein that in humans is encoded by the TP53 gene. The human p53 gene is mapped to chromosome 17. Human p53 is 393 amino acids long and has seven domains. It runs as a 53-kDa protein on SDS-PAGE. The pattern of p53 splicing was specificFor brain areas andFor individuals. Human kidney and heart express onlyFull-length p53. p53 plays a vital role in conserving stability by preventing genome mutation. ScienCells human p53 ELISA kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human p53-specific polyclonal antibodies are pre-coated onto 8 x 12 divisible strips. The human specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes to yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human p53 in the sample that is captured on the strips. Product Use This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures. Storage Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles. Shipping Shipped on gel ice Reference 1. Matlashewski G, Lamb P, Pim D, Peacock J, Crawford L, Benchimol S (December 1984). "Isolation and characterization of a human p53 cDNA clone: expression of the human p53 gene". EMBO J. 3 (13): 3257-62. 2. Isobe M, Emanuel BS, Givol D, Oren M, Croce CM (1986). "Localization of geneFor human p53 tumour antigen to band 17p13". Nature 320 (6057): 84-5. Kern SE, Kinzler KW, Bruskin A, Jarosz D,Friedman P, Prives C, Vogelstein B (June 1991). "Identification of p53 as a sequence-specific DNA-binding protein". Science (journal) 252 (5013): 1708-11. 3. McBride OW, Merry D, Givol D (1986). "The geneFor human p53 cellular tumor antigen is located on chromosome 17 short arm (17p13)". Proc. Natl. Acad. Sci. U.S.A. 83 (1): 130-134. 如需要其他产品资料请发邮件至wwwfudan@163.com索取
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