Description

Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.

ScienCells rat IL-10 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat IL-10-specific polyclonal antibodies are precoated onto 8 x 12 strips. The rat specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of rat IL-10 in the sample that is captured in the strips.

Product Use

This product is for research use only. It is not approved for use in humans, animals, or in vitro diagnostic procedures.

Storage

Store at 4°C For Frequent use, at -20°C For infrequent use. Avoid multiple Freeze-thaw cycles.

Shipping

Shipped on gel ice.

Reference

1. Alamartine, E.; Berthoux, P.; Mariat, C.; Cambazard, F.; Berthoux, F. Interleukin-10 promoter polymorphisms and susceptibility to skin squamous cell carcinoma after renal transplantation. J. Invest. Derm. 120: 99-103, 2003.
2. Grove, J.; Daly, A. K.; Bassendine, M. F.; Gilvarry, E.; Day, C. P. Interleukin 10 promoter region polymorphisms and susceptibility to advanced alcoholic liver disease. Gut 46: 540-545, 2000.
3. Eskdale, J.; Kube, D.; Tesch, H.; Gallagher, G. Mapping of the human IL10 gene and further characterization of the 5 prime flanking sequence. Immunogenetics 46: 120-128, 1997.
4. Gesser, B.; Leffers, H.; Jinquan, T.; Vestergaard, C.; Kirstein, N.; Sindet-Pedersen, S.; Jensen, S. L.; Thestrup-Pedersen, K.; Larsen, C. G. Identification of functional domains on human interleukin 10. Proc. Nat. Acad. Sci. 94: 14620-14625, 1997.
5. Asadullah, K.; Eskdale, J.; Wiese, A.; Gallagher, G.; Friedrich, M.; Sterry, W. Interleukin-10 promoter polymorphism in psoriasis. J. Invest. Derm. 116: 975-978, 2001.
6. Opdal, S. H.; Opstad, A.; Vege, A.; Rognum, T. O. IL-10 gene polymorphisms are associated with infectious cause of sudden infant death. Hum. Immun. 64: 1183-1189, 2003.
7. Kim, J. M.; Brannan, C. I.; Copeland, N. G.; Jenkins, N. A.; Khan, T. A.; Moore, K. W. Structure of the mouse Il-10 gene and chromosomal localization of the mouse and human genes. J. Immun. 148: 3618-3623, 1992.