Description
Type I collagen, a fibrous protein abundant in connective tissues including tendon, ligament, dermis and blood vessel, is the major component and the primary determinant of tensile strength of the extracelluar matrix (ECM). It is widely used as a thin layer on tissue-culture surfaces to enhance the attachment and proliferation of a variety of cells including endothelial cells, fibroblasts, hepatocytes, epithelial cells and etc. In addition, collagen I can self-assemble into a 3-D superamolecular gel in vitro, making it an ideal biological scaffold to promote more in vivo-like cellular morphology and function.
The ScienCell collagen I-Cell Culture Surface Coating Kit includes collagen I purified from rat tail tendon by a modification of the method of Bell et al and supplied as a sterile liquid in 1/1000 acetic acid. The kit also includes a 100x collagen I solvent, which can be used to dilute collagen I into appropriate concentration for the coating of cell culture vessels.
Product Use
This assay kit is used to coat cell culture surface in vitro. It is for research use only. Not for use in animals, humans, or diagnostic procedures.