Used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts in large volumes. - Easy-to-use: Simply drop a quick-dissolving tablet into your buffer.
- Complete protection: Instantly protect your proteins against a broad range of proteases.
- Flexible: Performs in extracts from almost any tissue or cell, including animals, plants, yeast, bacteria, or fungi.
- Safe: Non-toxic inhibitors without risk to you or those around you.
Starting concentration: 1 tablet contains protease inhibitors sufficient for a 50-ml cell extract.
Specificity: Mixture of several protease inhibitors with broad inhibitory specificity. For the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts.
Solubility/Stability: Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 25x stock solutions in 2 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1 to 2 weeks at +2 to +8°C or at least 12 weeks at -15 to -25°C. Can be used in thiol-containing solutions at room temperature.
Note: Inhibition efficiency is quickly and sensitively tested with the Universal Protease Substrate (Cat. Nos. 11 080 733 001 or 11 734 334 001). Performance-tested with pancreas extract. |