英文名称： AKAP10 Validated Chimera RNAi
- Specification
- Product Description:
- Homo sapiens A kinase (PRKA) anchor protein 10 (AKAP10), nuclear gene encoding mitochondrial protein, mRNA.
- Reactivity:
- Human
- Storage Instruction:
- Store at -20°C, do not exceed 4 - 5 freeze-thaw cycles to ensure product integrity.
- Supplied Product:
- DEPC water
- Target Refseq:
- NM_007202
- Note:
- Position of the Chimera RNAi.
-
- Publication Reference
- 1.
- dsCheck: highly sensitive off-target search software for double-stranded RNA-mediated RNA interference.
Naito Y, Yamada T, Matsumiya T, Ui-Tei K, Saigo K, Morishita S.Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W589-91.
- 2.
- Functional dissection of siRNA sequence by systematic DNA substitution: modified siRNA with a DNA seed arm is a powerful tool for mammalian gene silencing with significantly reduced off-target effect.
Ui-Tei K, Naito Y, Zenno S, Nishi K, Yamato K, Takahashi F, Juni A, Saigo K.Nucleic Acids Res. 2008 Apr;36(7):2136-51. Epub 2008 Feb 11.
- 3.
- Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference.
Ui-Tei K, Naito Y, Takahashi F, Haraguchi T, Ohki-Hamazaki H, Juni A, Ueda R, Saigo K.Nucleic Acids Res. 2004 Feb 9;32(3):936-48. Print 2004.
- 4.
- siDirect: highly effective, target-specific siRNA design software for mammalian RNA interference.
Naito Y, Yamada T, Ui-Tei K, Morishita S, Saigo K.Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W124-9.
- Applications
- RNAi Knockdown (Antibody validated)
- Western blot analysis of AKAP10 over-expressed 293 cell line, cotransfected with AKAP10 Validated Chimera RNAi ( Cat # H00011216-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AKAP10 monoclonal antibody (M04), clone 8C10 (Cat # H00011216-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 11216
- Gene Name:
- AKAP10
- Gene Alias:
- D-AKAP2,MGC9414,PRKA10
- Gene Description:
- A kinase (PRKA) anchor protein 10
- Gene Ontology:
- Hyperlink
- Gene Summary:
- The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein interacts with both the type I and type II regulatory subunits of PKA; therefore, it is a dual-specific AKAP. This protein is highly enriched in mitochondria. It contains RGS (regulator of G protein signalling) domains, in addition to a PKA-RII subunit-binding domain. The mitochondrial localization and the presence of RGS domains may have important implications for the function of this protein in PKA and G protein signal transduction. [provided by RefSeq
- Other Designations:
- A-kinase anchor protein 10,dual-specificity A-kinase anchoring protein 2,mitochondrial A kinase PPKA anchor protein 10,protein kinase A anchoring protein 10
- Related Disease
- Adenocarcinoma
- Arrhythmias, Cardiac
- Breast cancer
- Breast Neoplasms
- Colorectal Neoplasms
- Diabetes Mellitus, Type 2
- Esophageal Neoplasms
- Genetic Predisposition to Disease
- Hypercholesterolemia
- Hypertension
- Kidney Failure, Chronic
- Long QT Syndrome
- Metabolic Syndrome X
- Myocardial Infarction
- Renal Insufficiency, Chronic
- Werner syndrome