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Quick (2 minute) recovery of ultra-pure DNA from PCR, endonuclease digestions, cell lysates, etc. | | • | Column design allows DNA to be eluted at high concentrations into minimal volumes of solvent. | | • | Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc. | | • | Omits the use of organic denaturants as well as proteinases.
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| SPECIFICATIONS
| • | DNA Purity: Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. | | • | DNA Size Limits: From 75 bp to 23 kb. | | • | DNA Recovery: Typically, up to 25 µg total DNA can be eluted into as little as 25 µl water. For DNA 75 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%. | | • | Sample Sources: DNA from PCR, restriction endonuclease digestions, plasmid preparations, kinase reactions, etc. | | • | Product Detergent Tolerance: ≤ 5% Triton X-100, ≤ 5% Tween-20, ≤ 5% Sarkosyl, ≤ 0.1% SDS. | | • | Stability of Product Reagents: Integrity of kit components is guaranteed for up to one year from date of purchase. | | • | Quality Control: Reagents are routinely tested on a lot-to-lot basis to ensure they provide maximal performance and reliability. | |
| DESCRIPTION
Zymo Research’s DNA Clean & Concentrator-25™ product provides a hassle-free method for the rapid purification and concentration of up to 25 µg of high quality DNA from PCR, endonuclease digestions, or DNA from cell lysates. Simply add the specially formulated DNA Binding Buffer to your sample and transfer to the supplied Zymo-Spin™ Column. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin column technology to yield high quality, purified DNA in just minutes. DNA purified using the DNA Clean & Concentrator-25™ product is suitable for nucleotide sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.
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ORDERING INFORMATION:
| Products | Description | Protocol | Cat No. | Size | Price （ＲＭＢ） | | DNA Clean &
Concentrator™-25
| For 50 DNA preps. (uncapped columns) | | D4005 | 1 kit | | | | For 200 DNA preps. (uncapped columns) | | D4006 | 1 kit | | | | For 50 DNA preps. (capped columns) | | D4033 | 1 kit | | | | For 200 DNA preps. (capped columns) | | D4034 | 1 kit | | | | | |