HIGHLIGHTS • Simple, rapid recovery of ultra-pure DNA from large-scale sample sources. • Column design allows DNA to be eluted at high concentrations into minimal volumes of water or TE buffer. Unique column construction allows sample washing to be performed using a centrifuge or vacuum source. • Eluted DNA is well suited for use in PCR, DNA sequencing, DNA ligation, endonuclease digestion, RNA transcription, radiolabeling, etc.

SPECIFICATIONS  • Format: Spin Column. • DNA Purity: Highly purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. • DNA Size Limits: From 50 bp to 23 kb. • DNA Recovery: Typically, ≤ 500 µg total DNA can be eluted with ≥ 1 ml water or low salt elution buffer. For DNA 50 bp to 10 kb the recovery is 70-95%. For DNA 11 kb to 23 kb the recovery is 50-70%. • Sample Sources: DNA from large-scale sample sources including restriction endonuclease digestions and "crude" DNA preparations from cell-free lysates. • Product Detergent Tolerance: ≤ 5% Triton X-100, ≤ 5% Tween-20, ≤ 5% Sarkosyl, ≤ 0.1% SDS. • Equipment: Microcentrifuge and centrifuge, vacuum source, or syringe.

DESCRIPTION The DNA Clean & Concentrator™-500 was designed for the rapid, large format purification and concentration of up to 500 µg of high quality DNA from samples including large-scale restriction endonuclease digestions and "crude" DNA preparations. The DCC™-500 features a single buffer system that allows for efficient DNA adsorption onto the matrix of the supplied Zymo-Spin™ column. The DNA is washed twice using a centrifuge or vacuum source then eluted with a small volume (≥ 1 ml) of water using a centrifuge. The procedure is easy: simply add the specially formulated DNA Binding Buffer to your sample then transfer the mixture to the supplied to your sample then transfer the mixture to the supplied Zymo-Spin™ column. There is no need for organic denaturants or chloroform. Instead, the product features unique column technology to yield high quality, purified DNA in just minutes. DNA purified using the DCC™-500 is suitable for DNA sequencing, DNA transfection, RNA transcription, microarray analysis, PCR, nucleotide blotting, restriction endonuclease digestion, etc.

ORDERING INFORMATION: Products Description Protocol Cat No. Size    Price   (RMB)   DNA Clean & Concentrator™-500 For 10 DNA preps. PDF D4031 1 kit     For 20 DNA preps. PDF D4032 1 kit