美国Brand,null 产品货号： d2004Item#, Zymoprep™ 酵母质粒抽提试剂盒II<br>英文名称： Zymoprep™ Yeast Plasmid Miniprep II

Highlights

♦ Simple spin column format for plasmid rescue from yeast.

♦ Highly efficient.

♦ No glass beads, no phenol, no vortexing, no precipitation.

♦ Ideal for low copy and hard to isolate plasmids.

Specificity: S. cerevisiae, C. albicans and S. pombe, and other fungi species sensitive

to yeast lytic enzymatic digestion (Zymolyase™).

• Plasmid Purity: Plasmid is recovered in water or TE.

• Recovery Volume: 10 ul

• Plasmid Size: Up to 23 Kb (For plasmids > 15 Kb add elution water or TE and incubate for up to 5 minute before

centrifugation).

• DNA Binding Capacity: 5 ug

• Stability: Quality is guaranteed for 1 year from the purchase date.

The OneStep™ and OneStep-96™ PCR Inhibitor Removal Kits contain all the components necessary to efficiently remove contaminants from DNA and RNA preparations that can inhibit downstream enzymatic reactions such as PCR and RT, respectively. The column/plate matrices have been specifically designed for the efficient removal of polyphenolic compounds, humic/fulvic acids, tannins, melanin, etc. Polyphenolic compounds can contaminate impure DNA and RNA preparations from soil, plants, skin, and fecal samples amongst others. Sample cleanup is as simple as: applying, spinning and recovering a sample from the column or plate.

DNA is efficiently amplified by PCR following humic acid removal with the OneStep™ PCR Inhibitor Removal Kit. The figure shows amplification of a 200 bp product from DNA containing humic acid that was “treated” with the kit. Alternatively, PCR amplification was completely inhibited in the case of the “non-treated” sample. In each case, equal amounts of DNA were used for each PCR and equivalent amounts of the reaction were then analyzed in a 2.0% (w/v) agarose/TAE/EtBr gel. The ladder is a 100 bp DNA marker (Zymo Research). Hot start PCR was performed using ZymoTaq™ PreMix (Zymo Research).

DESCRIPTION

Zymo Research’s ZR-96 DNA Clean-up Kit™ provides a hassle-free method for rapid, large-scale (96 sample) purification and concentration of high-quality DNA from PCR samples, endonuclease digestions, or “crude” plasmid preparations. Simply add the specially formulated DNA Binding Buffer to your samples and transfer to the wells of the supplied ZR-96 Silicon-A Plate™. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin™ column technology to yield high quality, purified DNA in just minutes. DNA purified using the ZR-96 DNA Clean-up Kit™ is suitable for nucleotide sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.

Zymo Research’s ZR-96 DNA Clean-up Kit™ provides a hassle-free method for rapid, large-scale (96 sample) purification and concentration of high-quality DNA from PCR samples, endonuclease digestions, or “crude” plasmid preparations. Simply add the specially formulated DNA Binding Buffer to your samples and transfer to the wells of the supplied ZR-96 Silicon-A Plate™. There is no need for organic denaturants or chloroform. Instead, the product features Fast-Spin™ column technology to yield high quality, purified DNA in just minutes. DNA purified using the ZR-96 DNA Clean-up Kit™ is suitable for nucleotide sequencing, microarray analysis, PCR, nucleotide blotting, and restriction endonuclease digestion procedures.