| NucleoSpin® TriPrep | RNA, DNA and protein isolation | | | | Features | | | | Parallel isolation of RNA, DNA, and protein from undivided samples |  • Convenient one-column preparation of RNA, DNA, and protein
• High quality RNA and DNA, ready to use for downstream applications
• High protein yield independent of protein size, localization, modification, etc.
• Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
• Complete kit, including: NucleoSpin® Filters (shredders) for optimal lysis,
rDNase for on-column DNA digestion, Protein Solving Buffer for solving all types of proteins | | | | | Technology | Silica-membrane technology | | Format | Mini spin columns | | Sample material | < 5 x 106 cells, <30 mg human/ animal Tissue, <100 mg plant | | | RNA | DNA | Protein | | Fragment size | > 200 nt | < 30 kbp | 15–300 kbp | | Typical yield | < 70 µg | < 6 µg | < 1200 µg | | A260/A280 | 1.9–2.1 | 1.7–1.9 | – | | Typical RIN (RNA integrity number) | > 9 | – | – | Elution volume (RNA and DNA)
Resolubilization volume (protein) | 40–120 µL | 100 µL | 10–100 µL | | Preparation time | 30 min/6 preps | 35 min/6 preps (RNA+DNA) | 30 min/6 preps | | Binding capacity | 200 µg | 10 µg* | – | | | | Applications** | | | • Rapid purification of total RNA, DNA, and protein from small and precious samples – no sample splitting for
different isolations necessary
• Gene expression profiling, analysis of transgenic organisms, drug screening, genotyping
• Reliable interpretation of RNA, DNA, and protein amounts
• Good preservation of protein primary structure and posttranslational modifications (e.g., protein
phosphorylation) allows protein analysis without additional inhibitors (e.g., proteinase or phosphatase
inhibitors)
• Suitable for a broad spectrum of starting materials, including cultured cells, tissue, bacteria, yeast, and
plants
• RNA and DNA are ready to use for all typical downstream applications
• Proteins are ready to use for SDS-PAGE / Western blotting | | | * Binding capacity of DNA < 10 μg, strongly depending on RNA amount bound to the membrane.
** Kits to be used for research purposes only. DISTRIBUTION AND USE IN THE USA IS PROHIBITED FOR PATENT REASONS. | | | |
| | Ordering information | Product | Preps | Specification | REF | | NucleoSpin® TriPrep | 10 | NucleoSpin® TriPrep Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 ml), NucleoSpin® Filters, buffers, RNase-free rDNase | 740966.10 | | | 50 | as above | 740966.50 | | | 250 | as above | 740966.250 | | |
