一步式细菌DNAout
英文名称： One-Step Bacterial DNAout
型号:null    产品货号：  tz60806-50
价格:请致电：010-57128832,18610462672
品牌: 国产

  一步式细菌DNAout      One-Step Bacterial DNAout 50 次 60806-50

style="font-size: 20px; font-weight: bold; ">NucleoSpin® TriPrep

RNA, DNA and protein isolation

  Features   Parallel isolation of RNA, DNA, and protein from undivided samples • Convenient one-column preparation of RNA, DNA, and protein
• High quality RNA and DNA, ready to use for downstream applications
• High protein yield independent of protein size, localization, modification, etc.
• Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
• Complete kit, including: NucleoSpin® Filters (shredders) for optimal lysis, 
   rDNase for on-column DNA digestion, Protein Solving Buffer for solving all types of proteins
   
Technology Silica-membrane technology
Format Mini spin columns
Sample material

< 5 x 106 cells, <30 mg human/ animal Tissue, <100 mg plant
  RNA DNA Protein
Fragment size > 200 nt < 30 kbp 15–300 kbp
Typical yield < 70 µg < 6 µg < 1200 µg
A260/A280

1.9–2.1

 1.7–1.9 –
Typical RIN (RNA integrity number) > 9 – –
Elution volume (RNA and DNA)
Resolubilization volume (protein)		 40–120 µL 100 µL 10–100 µL
Preparation time 30 min/6 preps 35 min/6 preps (RNA+DNA) 30 min/6 preps
Binding capacity 200 µg 10 µg* –
 

Applications**
 
• Rapid purification of total RNA, DNA, and protein from small and precious samples – no sample splitting for
   different isolations necessary
• Gene expression profiling, analysis of transgenic organisms, drug screening, genotyping
• Reliable interpretation of RNA, DNA, and protein amounts
• Good preservation of protein primary structure and posttranslational modifications (e.g., protein
   phosphorylation) allows protein analysis without additional inhibitors (e.g., proteinase or phosphatase 
   inhibitors)
• Suitable for a broad spectrum of starting materials, including cultured cells, tissue, bacteria, yeast, and 
   plants
• RNA and DNA are ready to use for all typical downstream applications
• Proteins are ready to use for SDS-PAGE / Western blotting
 
* Binding capacity of DNA < 10 μg, strongly depending on RNA amount bound to the membrane.
** Kits to be used for research purposes only. DISTRIBUTION AND USE IN THE USA IS PROHIBITED FOR PATENT REASONS.
  

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Ordering information

Product

Preps

 Specification REF
NucleoSpin® TriPrep

10

 NucleoSpin® TriPrep Columns with Collection Tubes, Collection Tubes (2 mL), Collection Tubes (1.5 ml), NucleoSpin® Filters, buffers, RNase-free rDNase 740966.10
 

50

 as above 740966.50
 

250

 as above 740966.250