HYQ 柱式法植物RNA试剂盒 Plant RNA Kit(HG40702-50)
英文名称： Plant RNA Kit
型号:null    产品货号： HG40702-50
价格:请致电：010-57128832,18610462672
品牌: 美国

"center">minutesBinding capacityUp to 100 µg RNAYield* from 10 mg tissueHeart: 8–12 µg
Esophagus: 8–12 µg
Muscle: 5–10 µg
Skin: 4–8 µgElution volume30–100 µl

* Amounts can vary due to species, developmental stage, etc. Since the RNeasy procedures enrich for mRNA and other RNA species >200 nucleotides, the total RNA yield does not include 5S rRNA, tRNA, and other low-molecular-weight RNAs, which make up 15-20% of total cellular RNA.

RNeasy technology simplifies total RNA isolation by combining the stringency of guanidine-isothiocyanate lysis with the speed and purity of silica-membrane purification. There is no need for centrifugation through CsCl cushions or precipitation with LiCl or alcohol. RNeasy sample preparation technology is fully licensed, allowing RNeasy purified nucleic acids to be used in any molecular assay or other downstream application without risk of patent infringement.

The convenient RNeasy Fibrous Tissue protocol integrates proteinase K and RNase-free DNase digestion steps during RNA isolation to break down proteins and genomic DNA. This ensures easy and efficient isolation of high-quality total RNA (see figure "High-quality RNA from fiber-rich tissues") and higher yields of RNA from fiber-rich tissues compared with other silica-based methods (see table "Highest RNA yields using RNeasy Fibrous Tissue Kits").

Highest RNA yields using RNeasy Fibrous Tissue Kits*

Rat tissue (10 mg)RNA yield (µg) with RNeasy Fibrous Tissue Mini KitRNA yield (µg) with silica kit (Supplier AIV)RNA yield (µg) with silica kit (Supplier R)RNA yield (µg) with silica kit (Supplier S)
Heart8.23.5<0.52.5
Muscle5.30.52.0†2.2
Skin6.0Not determinedNot determinedNot determined
Esophagus13.9Not determinedNot determinedNot determined

* RNA was isolated from the tissues indicated following kit suppliers specifications. Amounts can vary due to species, developmental stage, etc.
† Columns clogged, resulting in poor-quality RNA. This yield may be overestimated due to contaminants in the prep.

Procedure

Samples are lysed in a guanidine-isothiocyanate buffer. After dilution of the lysate, the sample is treated with proteinase K. Debris is pelleted by centrifugation. Ethanol is then added to the cleared lysate, and RNA is bound to the RNeasy silica membrane. Traces of DNA that may copurify are removed by a DNase treatment on the RNeasy spin column. DNase and any contaminants are washed away, and high-quality total RNA is eluted in RNase-free water.

Applications

RNeasy Fibrous Tissue Kits provide RNeasy quality total RNA, suitable for any downstream application, such as array analysis and real-time RT-PCR (see figures "Real-time gene expression analysis in a variety of fibrous tissues" and "LightCycler analysis of high-quality RNA from heart").

A range of optimized kits for RNA purification from tissue samples are available. Use the ProductFinder to choose the right kit.