EZ Bisulfite DNA Clean-up Kit™  HIGHLIGHTS • Consistent, high yield recovery of bisulfite-treated DNA from any “homebrew” or commercial reaction mixture containing bisulfite. • Simple spin column procedure with small elution volumes (≥ 10 µl) for concentrated DNA. • Recovered DNA is ideal for downstream methylation analysis including PCR, endonuclease digestion, sequencing, arrays, etc.

SPECIFICATIONS   • DNA Input: Up to 5 µg DNA (per column) from “homebrew” or commercial reaction mixtures containing bisulfite. • DNA Recovery: Typical yields are between 80-90%. • Elution: DNA can be eluted with ≥ 10 µl M-Elution Buffer or water. • Detergence Tolerance : ≤ 5% Triton X-100, ≤ 5% Tween-20, ≤ 5% Sarkosyl, ≤ 0.1% SDS

DESCRIPTION The EZ Bisulfite DNA Clean-up Kit™ has been specifically designed for the purification of bisulfite-treated DNA from any “homebrew” or commercial reaction mixture containing bisulfite. The product features innovative Fast-Spin, in-column desulphonation and wash technologies that eliminate DNA loss, buffer carryover and the need for ethanol/isopropanol precipitations. The procedure is easy and DNA clean-up can be completed in just minutes. Bisulfite-treated DNA purified with the EZ Bisulfite DNA Clean-up Kit™ is ideal for PCR amplification for downstream DNA methylation analysis including endonuclease digestion, sequencing, microarrays, etc.   Overview of EZ Bisulfite DNA Clean-up Kit™ Procedure   ORDERING INFORMATION: Products Protocol Cat No. Size Price （ＲＭＢ）   EZ Bisufite DNA Clean-up Kit™ PDF D5025 50 preps.     PDF D5026 200 preps.     EZ-96 Bisufite DNA Clean-up Kit™ (Shallow-Well) PDF D5027 2x96 preps.     EZ-96 Bisufite DNA Clean-up Kit™ (Deep-Well) PDF D5028 2x96 preps.