抗Tubulin-Alpha鼠单克隆抗体
英文名称： Mouse anti alpha Tubulin Monoclonal antibody
型号:null    产品货号： 60004-1-Ig
价格:请致电：010-57128832,18610462672
品牌: 美国
试剂级别： 细胞培养级

 拓然生物经典产品：

Applications
Tested applications ELISA, IHC, IF, WB
Cited applications WB
Species specificity Human,Mouse,Rat; other species not tested.
Cited species Human
Positive WB detected in Multi-cells, A431 cells, HeLa, HeLa cells, L02 cells, SW 1990 cells
Positive IHC detected in Human liver cancer tissue, human testis tissue
Positive IF detected in Hela cells, MCF-7 cells
Recommended dilution WB: 1:500-1:5000
IHC: 1:50-1:200
IF: 1:20-1:200
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Images
Prev12NextView more validation data
  • 66031-1-Ig;multi-cellsWestern blot analysis of alpha-tubulin in various tissues and cell lines using Proteintech antibody 66031-1-Ig at a dilution of 1:10000.
  • 66031-1-Ig;HeLaWestern blot of Hela cell with anti-Tubulin-Alpha (66031-1-Ig) at various dilutions.
  • 66031-1-Ig;Hela cellsImmunofluorescent analysis of Hela cells, using TUBA1B antibody 66031-1-lg at 1:50 dilution and Rhodamine-labeled goat anti-mouse IgG (red).
  • 66031-1-Ig;human liver cancer tissueImmunohistochemical of paraffin-embedded human liver cancer using 66031-1-Ig(Tubulin-Alpha antibody) at dilution of 1:100 (under 40x lens)
  • 66031-1-Ig;human liver cancer tissueImmunohistochemical of paraffin-embedded human liver cancer using 66031-1-Ig(Tubulin-Alpha antibody) at dilution of 1:100 (under 10x lens)
Product Information
Source Mouse Purification method Protein A purification
Isotype IgG2b Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information
Immunogen Alpha Tubulin fusion protein ag18034 Full name Tubulin, alpha 1b
Calculated molecular
weight		 50kd Observed molecular 
weight		 52kD
GenBank accession 
number		 BC009314 Gene ID (NCBI) 10376
Gene symbol TUBA1B Synonyms Alpha Tubulin, Alpha tubulin ubiquitous, TUBA1B, Tubulin alpha, tubulin, alpha 1b
Background

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs. This antibody recognize Tubulin Alpha only.