Applications Tested Applications: | ELISA,WB,IHC,IF | Specificity Reaction: | Human, Mouse,Rat,Zebrafish,Pig; Other species are not tested.Please decide the specificity by homology | Positive IHC detected In: | H-kidney | Positive IF detected In: | HepG2 |
Application key: WB=Western Blotting ,IHC=Immunohistochemistry, IF=Immunofluorescence
MCF7 cell were subjected to SDS PAGE followed by western blot with 60008-1-Ig(ACTB Antibody) at dilution of 1:2000 Immunohistochemistry of paraffin-embedded H-kidney using 60008-1-Ig(ACTB Antibody) at Dilution 1:100 (under 10x lens) Immunohistochemistry of paraffin-embedded H-kidney using 60008-1-Ig(ACTB Antibody) at Dilution 1:100 (under 40x lens) Immunofluorescent analysis of HepG2 cells, using 60008-1-lg and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). Western blot of Actin-Beta (60008-1-Ig, 1:5000) in various tissues and cell lines. Western blot of Actin-Beta (60008-1-Ig) with Hela cell at various dilution.
Product Information Source: | Mouse | Purify: | Caprylic Acid/Ammonium Sulfate Precipitatio | IsoType: | IgM | Storage: | PBS with 0.1% Sodium Azide, 50% Glycerol, pH 7.3. -20oC, Avoid freeze / thaw cycles. |
Immunogen Information Immunogen: | ag0297 | Full Name: | actin, beta | Predicted Molecular Weight: | 375aa,42kd | Observed Molecular Weight: | 42kd | GenbankNo | BC002409 | GeneID: | 60 | Symbol | ACTB | Synonyms | ACTB;PS1TP5BP1 ;beta-actin; β-actin |
Handbooks http://www.ptglab.com/Products/Pictures/pdf/60008-1-Ig.pdf |