英文名称： gst Antibody
Applications
| Tested Applications: | ELISA,WB |
| Specificity Reaction: | Schistosoma japonicum; Other species are not tested.Please decide the specificity by homology |
| Positive WB detected In: | Recombinant protein |
Application key: WB=Western Blotting ,IHC=Immunohistochemistry, IF=Immunofluorescence
Images
Recombinant protein were subjected to SDS PAGE followed by western blot with 66001-1-Ig(gst Antibody) at dilution of 1:10000 - Western blot of GST-tag (66001-1-Ig) with GST recombinant protein at various dilution.
Product Information
| Source: | Mouse | Purify: | Antigen Affinity purified |
| IsoType: | IgG2a | Storage: | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20oC, Avoid freeze / thaw cycles. |
Immunogen Information
| Immunogen: | Recombinant Protein | Full Name: | |
| Caculated Molecular Weight: | 26kd | Observed Molecular Weight: | 28kd |
| GenbankNo | GeneID: | ||
| Symbol | Synonyms |
Background
Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. GST(Glutathione S-Transferase) is a widely used protein tag encoded by the Schistosoma japonicum. GST provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. This GST-fusion protein can then be purified from cells via its high affinity for glutathione. Antibodies to GST are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in cells. Handbooks |