Application:

For the detection of digoxigenin-labeled compounds.

• Dot Blot
• ELISA
• Immunohisto/cytochemistry
• In situ hybridization
• Northern blot
• Southern blot
• Western blot

Specificity: Polyclonal antibodies from sheep show 100% reactivity with digoxigenin and digoxin, but no cross-reactivity with other steroids, such as human estrogens (e.g., estradiol) or androgens (e.g., ***).
Working concentration:
Dot blot: 150 mU/ml
ELISA: 150 - 300 mU/ml
Immunohisto/cytochemistry: 250 - 500 mU/ml
In situ hybridization: 1.5 - 7.5 U/ml
Southern blot: 150 mU/ml
Western blot: 250 - 500 mU/ml

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.