DIG-High Prime 高效地高辛DNA标记试剂
英文名称： DIG-High Prime roche 11585606910
型号:null    产品货号： 11585606910
价格:请致电：010-57128832,18610462672
品牌: roche

DIG-High Prime 高效地高辛DNA标记试剂，标记DNA探针, 只需一步操作, 标记效率较通常随机引物法高3倍。40 T

Application

DIG-High Prime is used for the highly efficient random-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile.
DIG-labeled probes are generated at high yield within one hour or after overnight incubation.
The standard reaction with 1 µg template DNA will routinely give a yield of 0.8 µg DIG-labeled DNA after 1 hour, and 2.3 µg after a 20-hour incubation.
DIG-High Prime labeled DNA probes are used in Southern, northern, and dot blots, as well as in colony or plaque hybridization.
Due to highly specific and sensitive detection systems, DIG-labeled DNA probes can be used for single-copy gene detection in 1 µg total human DNA.
The use of the alkali-labile form of DIG-dUTP, in which the digoxigenin moiety is connected to the spacer arm via an alkali-labile ester bond (Figure 1), enables easier and more efficient stripping and reprobing of blots.

Benefits

DIG-High Prime guarantees efficient labeling of:
  • DNA amounts ranging from 10 ng to 3 µg in a standard reaction.
  • DNA of different lengths ranging from small restriction fragments to l or cosmid DNA.
  • DNA, supercoiled or linearized.
  • DNA in low melting-point agarose.

Product Description

Labeling efficiency: A standard labeling reaction with 1 µg template yields 0.8 µg newly synthesized digoxigenin-labeled DNA after 1 hour, and 2 µg after a 20-hour incubation at 37°C.

Background Information

DIG-High Prime, a novel 5x conc. labeling mixture contains optimal concentrations of random primers, nucleotides, and DIG-dUTP, alkali-labile, in a highly efficient reaction buffer with Klenow enzyme. Simply add DIG-High Prime to the denatured template DNA. The number of pipetting steps are thus reduced to a minimum, while reproducibility and accuracy of experiments are effectively increased.
DIG-labeled probes in the reaction mix or in the hybridization buffer are stable for more than 12 months stored at -15 to -25°C. They can be reused several times if freshly denatured before use.
 


Figure 1: Structure of alkali-labile DIG-11-dUTP.

Contents

5x conc. labeling mixture, 160 µl containing random primer mixture, 1 U/µl Klenow enzyme, labeling grade, 1 mM dATP, 1 mM dCTP, 1 mM dGTP, 0.65 mM dTTP, 0.35 mM DIG-11-dUTP, alkali-labile, and 5x stabilized reaction buffer in glycerol, 50 (v/v).

Typical Experiment



Table 1: Yield of DIG-High Prime labeling reaction.

Quality

Function test: In a standard assay with 1 µg linearized pBR 328, 0.8 µg of DIG-labeled DNA is synthesized after 1 hour, and 2.3 µg after 20 hours. When this labeled DNA is used for hybridization at a concentration of 20 ng/ml, 0.03 pg homologous DNA are detected by chemiluminescence with the anti-DIG-alkaline phosphatase conjugate and CSPD on a dot or Southern blot.

其他相关产品：

名称

货号

产品

规格

价格￥

DIG DNA Labeling and Detection Kit地高辛DNA标记和检测试剂盒

11093657910             

Roche        

Kit      

7795    

DIG-11-dUTP随机引物法标记DNA, 碱性***酸酶(AP)生色反应检测, 标记探针用于Southern、Northern和斑点杂交以及菌落和噬菌体筛选。组成: 未标记对照DNA1; 未标记对照DNA2; DNA稀释液; DIG-标记对照DNA; 六核苷酸混合物; Klenow 2u/μl; 封闭液; BCIP/NBT; 10×dNTP标记混合物; 抗-地高辛-碱性***酸酶复合物。一个Kit可用于25次标记反应和50次检测(100cm2膜), -20℃保存。

DIG-High Prime DNA Labeling and

Detection Starter Kit I

高效DNA地高辛标记和检测试剂盒I

11745832910      

Roche

Kit

4872

比普通DIG随机引物法标记效率高, 操作简单, 可用于Southern、Northern和斑点杂交以及菌落和噬菌体筛选。一个Kit可用于12次标记反应和24次检测反应。组成: 5×DIG-高效标记试剂; 未标记对照DNA, PBR328; DNA稀释液; 抗-DIG-碱性***酸酶复合物; NBT/BCIP储存液; 10×封闭液; DIG定量复试纸条; DIG对照试纸条, -20℃保存。

DIG-High Prime DNA Labeling and

Detection Starter Kit II

高效DNA地高辛标记和检测试剂盒II

11585614910

Roche

Kit

4998

比普通DIG随机引物法标记效率高, 采用CSPD化学发光检测灵敏度更高。一个Kit可用于12次标记反应和24次检测反应。组成: 5×高效DIG标记复合物; 未标记对照DNA, PBR328; DNA稀释液; 抗-DIG-碱性***酸酶连接物; CSPD; 10×封闭液; DIG定量试纸条; DIG对照试纸条, NBT/BCIP储存液; -20℃保存。

DIG DNA Labeling Kit

地高辛DNA标记试剂盒

11175033910

Roche

40T

6922

随机引物扩增法以DIG-11-dUTP标记DNA; DIG标记探针可用于各种膜杂交。

DIG Nucleic acid Detection Kit

地高辛核酸检测试剂盒

11175041910

Roche

Kit

4612

NBT/BCIP显色法检测, 灵敏度可达0.1pg同源DNA; 可检测40张100cm2杂交膜。-20℃保存。

DIG Oligonucleotide 3’-End Labeling Kit

寡核苷酸3’末端地高辛标记试剂盒

3353575910

Roche

25T

6636

通过末端转移酶以DIG-11-ddUTP标记14-100bp的寡核苷酸探针3’端用于各种膜杂交和原位杂交。每个反应可标记100pmol的寡聚核苷酸。

DIG Oligonucleotide Tailing Kit

寡核苷酸加尾地高辛标记试剂盒

3353583910

Roche

25 T

5594

通过末端转移酶以DIG-11-ddUTP在寡核苷酸探针3’端加一平均长度50bp(10-100bp)的寡核苷酸, 用于DNA/RNA膜杂交和原位杂交。每个反应可标记100pmol的寡聚核苷酸。

DIG RNA Labeling Kit (SP6/T7)

地高辛RNA标记试剂盒(SP6/T7)

11175025910

Roche

2´10T

6779

由SP6或T7 RNA聚合酶通过转录反应过程标记RNA, 1mg模板可产生约10mg标记RNA用于杂交。-20℃保存。组成: pSPT18 DNA, pSPT19 DNA, 对照DNA1, 对照DNA2, DIG标记对照RNA, NTR标记混合物, 转录缓冲液, RNase抑制剂, 无RNase的DNase I, SP6 RNA聚合酶, T7 RNA聚合酶。

PCR DIG Probe Synthesis Kit

PCR地高辛探针合成试剂盒

11636090910

Roche

25T

4998

标记的探针可在Southern杂交后从5-10mg的人基因组DNA检测出单拷贝基因, 是从少量DNA模板制备大量探针的最有效、最经济的方法。

DIG Northern Starter Kit

12039672910

Roche

10T

4511

包含标记DIG RNA及进行Northern印迹的所有必需试剂, 配合DIG Wash and Block Buffer Set方便进行Northern印迹; 能作10个标记反应及10次检测(100mm2膜)。特别适合初用者使用; 标记混合物经最优化, 配合提供的化学发光底物CDP-Star, 能产生最高的灵敏度。

DIG Luminescent Detection Kit

地高辛发光检测试剂盒

 11363514910

Roche

Kit

5023

CSPD化学发光法检测, 灵敏度可达0.03pg同源DNA; 可检测50张100cm2杂交膜。

DIG High Prime

高效地高辛DNA标记试剂

11585606910

Roche

40T

6997

标记DNA探针, 只需一步操作, 标记效率较通常随机引物法高3倍。40 T