DIG-High Prime DNA Labeling and Detection Starter Kit I 高效DNA地高辛标记和检测试剂盒I ROCHE 现货

DIG-High Prime DNA Labeling and Detection Starter Kit I

Kit for color detection with NBT/BCIP

Application

DIG-High Prime labeled probes can be used in Southern-, northern- and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and color detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering a ready-made blocking solution, combined stock solution of NBT/ BCIP, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

Benefits

The High Prime method of labeling offers many benefits. Plasmid, cosmid, and lambda phage DNA have all been used as templates. We have achieved equal efficiency when labeling 10 ng to 3 µg of template DNA, which may be linear or supercoiled.

Product Description

Sensitivity and specificity: The standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 hour of labeling, and 2.3 µg labeled DNA after a 20-hour reaction. In a dot-blot hybridization, 0.1 pg of homologous DNA is detectable after a 16-hour color development (probe concentrations at 20 ng/ml). A single-copy human gene (tPA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.

Contents

1.        DIG-High Prime, 5x conc., 50 µl

2.        DIG-labeled Control DNA, 20 µl, (5 µg/ml), pBR328 (linearized with Bam HI)

3.        DNA Dilution Buffer, 3 x 1 ml

4.        Anti-digoxigenin-AP Conjugate, 100 µl

5.        NBT/BCIP Stock Solution, conc., 6 x 1 ml

6.        Blocking Solution, 10x conc., 4 x 100 ml

7.        DIG Easy Hyb Granules, 4 x 100 ml

Roche地高辛标记及检测相关试剂盒

名称	编号	产地		规格	价格￥
DIG DNA Labeling and Detection Kit 地高辛DNA标记和检测试剂盒	11093657910	Roche		Kit	8732
	DIG-11-dUTP随机引物法标记DNA, 碱性***酸酶(AP)生色反应检测, 标记探针用于Southern、Northern和斑点杂交以及菌落和噬菌体筛选。组成: 未标记对照DNA1; 未标记对照DNA2; DNA稀释液; DIG-标记对照DNA; 六核苷酸混合物; Klenow 2u/μl; 封闭液; BCIP/NBT; 10×dNTP标记混合物; 抗-地高辛-碱性***酸酶复合物。一个Kit可用于25次标记反应和50次检测(100cm2膜), -20℃保存。
DIG-High Prime DNA Labeling and Detection Starter Kit I 高效DNA地高辛标记和检测试剂盒I	11745832910	Roche		Kit	5457
	比普通DIG随机引物法标记效率高, 操作简单, 可用于Southern、Northern和斑点杂交以及菌落和噬菌体筛选。一个Kit可用于12次标记反应和24次检测反应。组成: 5×DIG-高效标记试剂; 未标记对照DNA, PBR328; DNA稀释液; 抗-DIG-碱性***酸酶复合物; NBT/BCIP储存液; 10×封闭液; DIG定量复试纸条; DIG对照试纸条, -20℃保存。
DIG-High Prime DNA Labeling and Detection Starter Kit II 高效DNA地高辛标记和检测试剂盒II	11585614910	Roche		Kit	5598
	比普通DIG随机引物法标记效率高, 采用CSPD化学发光检测灵敏度更高。一个Kit可用于12次标记反应和24次检测反应。组成: 5×高效DIG标记复合物; 未标记对照DNA, PBR328; DNA稀释液; 抗-DIG-碱性***酸酶连接物; CSPD; 10×封闭液; DIG定量试纸条; DIG对照试纸条, NBT/BCIP储存液; -20℃保存。
DIG DNA Labeling Kit 地高辛DNA标记试剂盒	11175033910	Roche		40T	7753
	随机引物扩增法以DIG-11-dUTP标记DNA; DIG标记探针可用于各种膜杂交。
DIG Nucleic acid Detection Kit 地高辛核酸检测试剂盒	11175041910	Roche		Kit	5165
	NBT/BCIP显色法检测, 灵敏度可达0.1pg同源DNA; 可检测40张100cm2杂交膜。-20℃保存。
DIG Oligonucleotide 3’-End Labeling Kit 寡核苷酸3’末端地高辛标记试剂盒	3353575910	Roche		25T	7432
	通过末端转移酶以DIG-11-ddUTP标记14-100bp的寡核苷酸探针3’端用于各种膜杂交和原位杂交。每个反应可标记100pmol的寡聚核苷酸。
DIG Oligonucleotide Tailing Kit 寡核苷酸加尾地高辛标记试剂盒	3353583910	Roche		25 T	6266
	通过末端转移酶以DIG-11-ddUTP在寡核苷酸探针3’端加一平均长度50bp(10-100bp)的寡核苷酸, 用于DNA/RNA膜杂交和原位杂交。每个反应可标记100pmol的寡聚核苷酸。
DIG RNA Labeling Kit (SP6/T7) 地高辛RNA标记试剂盒(SP6/T7)	11175025910	Roche	2´10T		7592
	由SP6或T7 RNA聚合酶通过转录反应过程标记RNA, 1mg模板可产生约10mg标记RNA用于杂交。-20℃保存。组成: pSPT18 DNA, pSPT19 DNA, 对照DNA1, 对照DNA2, DIG标记对照RNA, NTR标记混合物, 转录缓冲液, RNase抑制剂, 无RNase的DNase I, SP6 RNA聚合酶, T7 RNA聚合酶。