1.5ml离心管（Axygen）
型号:： Hy0022
价格:请致电：010-57128832,18610462672
品牌: 美国

easily and safely be detected (e.g ., with the DIG Nucleic Acid Detection Kit or the DIG Luminescent Detection Kit for Nucleic Acids). DIG-11-dUTP can replace dTTP in the following enzymatic procedures:nick translation (e.g., with E. coli DNA Polymerase I) random-primed DNA labeling (e.g ., with Klenow Enzyme) labeling during PCR (e.g., with Taq DNA polymerase) 3-tailing (with terminal transferase) cDNA synthesis (e.g., with Transcriptor or M-MuLV reverse transcriptase)The nucleotide is also a substrate for T4 and T7 DNA Polymerases.Note: When this nucleotide is subjected to 0.1 - 0.5 NaOH at room temperature, the DIG moiety will remain attached. It is therefore ideal for use in DIG-labeled DNA that needs to survive exposure to alkaline treatment. However, if you intend to make a DIG-labeled probe that can be removed by alkali denaturation (e.g ., during stripping and reprobing of blots), do not use this alkali-stable preparation. Instead, use the alkali-labile form of DIG-11-dUTP. Product DescriptionBrief description: Digoxigenin is bound to deoxyuridine triphosphate via an alkali-resistant ether linkage. Formula: C45H61N4O21P3Li4Molecular weight: Mr = 1090.7 (DIG-11-dUTP-Li4)Resistance to alkali: The preparation is stable in the presence of 0.1 - 0.5 M NaOH at room temperature.ContentsDIG-11-dUTP, tetralithium salt; 1 mM solutionQualityTypical analysis: at least 85% DIG-11-dUTP (HPLC, area %). NotesFor your convenience, DIG-dUTP is also available in:convenient labeling mixes (single solution with three conventional dNTPs and DIG-dUTP), and technique-specific kits (nucleotides, buffers, enzymes, and other reagents in separate vials).