迷你掌中宝离心机
型号:： LX300
价格:请致电：010-57128832,18610462672
品牌: 中国    产品商标: lx300

or after overnight incubation.The standard reaction with 1 g template DNA will routinely give a yield of 0.8 g DIG-labeled DNA after 1 hour, and 2.3 g after a 20-hour incubation.DIG-High Prime labeled DNA probes are used in Southern, northern, and dot blots, as well as in colony or plaque hybridization.Due to highly specific and sensitive detection systems, DIG-labeled DNA probes can be used for single-copy gene detection in 1 g total human DNA.The use of the alkali-labile form of DIG-dUTP, in which the digoxigenin moiety is connected to the spacer arm via an alkali-labile ester bond (Figure 1), enables easier and more efficient stripping and reprobing of blots.BenefitsDIG-High Prime guarantees efficient labeling of:DNA amounts ranging from 10 ng to 3 g in a standard reaction. DNA of different lengths ranging from small restriction fragments to l or cosmid DNA. DNA, supercoiled or linearized. DNA in low melting-point agarose. Product DescriptionLabeling efficiency: A standard labeling reaction with 1 g template yields 0.8 g newly synthesized digoxigenin-labeled DNA after 1 hour, and 2 g after a 20-hour incubation at 37°C.Background InformationDIG-High Prime, a novel 5x conc. labeling mixture contains optimal concentrations of random primers, nucleotides, and DIG-dUTP, alkali-labile, in a highly efficient reaction buffer with Klenow enzyme. Simply add DIG-High Prime to the denatured template DNA. The number of pipetting steps are thus reduced to a minimum, while reproducibility and accuracy of experiments are effectively increased.DIG-labeled probes in the reaction mix or in the hybridization buffer are stable for more than 12 months stored at -15 to -25°C. They can be reused several times if freshly denatured before use.Figure 1: Structure of alkali-labile DIG-11-dUTP. Contents5x conc. labeling mixture, 160 l containing random primer mixture, 1 U/l Klenow enzyme, labeling grade, 1 mM dATP, 1 mM dCTP, 1 mM dGTP, 0.65 mM dTTP, 0.35 mM DIG-11-dUTP, alkali-labile, and 5x stabilized reaction buffer in glycerol, 50% (v/v).Typical ExperimentTable 1: Yield of DIG-High Prime labeling reaction. QualityFunction test: In a standard assay with 1 g linearized pBR 328, 0.8 g of DIG-labeled DNA is synthesized after 1 hour, and 2.3 g after 20 hours. When this labeled DNA is used for hybridization at a concentration of 20 ng/ml, 0.03 pg homologous DNA are detected by chemiluminescence with the anti-DIG-alkaline phosphatase conjugate and CSPD on a dot or Southern blot.