蛋白质、核酸标记和检测 DIG标记及检测试剂盒 DIG(地高辛)DNA标记及检测试剂盒 DIG DNA labeling and Detection Kit：Random primed DNA labeling with digoxigenin-dUTP, alkali-labile and detection of hybrids by enzyme immunoassay 原理：用DIG-dUTP为底物，随机掺入法进行DNA标记，通过酶联免疫杂交的方法检测。该试剂盒可以标记25次反应，每次标记的DNA量为10ug-20ug，并可做50张10×10cm膜的杂交和检测。应用：非***性DNA标记及检测试剂盒以检测0.1pg的同源DNA，能检测1ug哺乳动物DNA中的单拷贝基因。跟***性标记系统比较，此试剂盒能快速得到实验结果，而且消除了***性同位素的不安全问题。从DNA标记和杂交能见到检测结果，整个实验过程24小时内能完成。这试剂盒的灵敏度与特异性使它适用于所有的杂交技术以替代***性同位素标记和***自显影术。这个标记方法和检测系统适用于DNA印迹转移、菌落杂交、噬斑杂交及原位杂交。 产品号 产品规格 厂商 价格 109365711093657910 Kit：1 kit (25 labeling reactions and detection of 50 blots) Roche 7447 DIG DNA Labeling and Detection KitApplicationRandom-primed labeling of DNA probes with DIG-11-dUTP, alkali-labile and color detection of DIG-labeled hybrids. DIG-labeled probes are used for hybridization in Southern, northern, and dot blots, as well as for colony and plaque screening.DIG-labeled hybrids are detected with an anti-DIG-alkaline phosphatase conjugate and the substrates NBT (nitroblue tetrazolium salt) and BCIP (5-bromo-4-chloro-3-indolyl phosphate, toluidinium salt), which give a light-blue precipitate.Product DescriptionSensitivity and specificity: The standard labeling reaction with 1 g template will give a yield of 260 ng DIG-labeled DNA after a 1-hour incubation at 37°C, or 780 ng after a 20-hour incubation. The sensitivity of DIG detection depends on the concentration of the DIG-labeled probe in the hybridization reaction, and the duration of the color reaction.Table 1: Sensitivity of DIG detection.Contents1.       Unlabeled Control DNA 1, 20 l, 100 g/ml 2.       Unlabeled Control DNA 2, 20 l, 200 g/ml 3.       DNA Dilution Buffer, 2 x 1 ml 4.       DIG-labeled Control DNA, 50 l, 5.2 g/ml 5.       10x Hexanucleotide Mix, 50 l 6.       10x dTP Labeling Mixture, 50 l 7.       Klenow Enzyme, labeling grade, 25 l, 2 U/l 8.       Anti-digoxigenin-AP-conjugate, 200 l, 750 U/ml 9.       NBT/BCIP Concentrated Stock Solution, 10 x 1 ml 10.    Blocking Reagent, 2 x 50 g