DIG-High Prime DNA Labeling and Detection Starter Kit II高效DNA地高辛标记和检测试剂盒II

Application

DIG-High Prime labeled probes can be used in northern, Southern-, and dot-blot analysis, as well as colony and plaque hybridizations. This kit offers random-primed labeling of DNA templates with DIG-11-dUTP, alkali-labile, and chemiluminescent detection of the DIG-labeled hybrids. This kit was assembled with convenience in mind, offering ready-to-use CSPD supplied with a dripping device for easy application, ready-made blocking solution, and DIG Easy Hyb granules. The DIG-High Prime mixture includes stabilized Klenow enzyme, nucleotides, primers, and reaction buffer, all in one convenient reagent.

Benefits

The High Prime method of labeling offers many benefits: in addition to the optimized labeling efficiency, plasmid, cosmid, and phage DNA, linearized or supercoiled can be used as templates, yielding the same labeling efficiency.

Product Description

Sensitivity and specificity: Standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 h of labeling, and 2.3 µg labeled DNA after 20 h reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.

Contents

1.        DIG-High Prime, 5x conc., 50 µl

2.        DIG-labeled Control DNA, 20 µl, (5 µg/ml) pBR328 (linearized with Bam HI)

3.        DNA Dilution Buffer, 3 x 1 ml

4.        Anti-digoxigenin-AP Conjugate, 50 µl

5.        CSPD, ready-to-use, 50 ml

6.        Blocking Solution, 10x conc., 4 x 100 ml

7.        DIG Easy Hyb Granules, 4 x 100 ml

Roche地高辛标记及检测相关试剂盒

名称	编号	产地		规格	价格￥
DIG DNA Labeling and Detection Kit 地高辛DNA标记和检测试剂盒	11093657910	Roche		Kit	8732
	DIG-11-dUTP随机引物法标记DNA, 碱性***酸酶(AP)生色反应检测, 标记探针用于Southern、Northern和斑点杂交以及菌落和噬菌体筛选。组成: 未标记对照DNA1; 未标记对照DNA2; DNA稀释液; DIG-标记对照DNA; 六核苷酸混合物; Klenow 2u/μl; 封闭液; BCIP/NBT; 10×dNTP标记混合物; 抗-地高辛-碱性***酸酶复合物。一个Kit可用于25次标记反应和50次检测(100cm2膜), -20℃保存。
DIG-High Prime DNA Labeling and Detection Starter Kit I 高效DNA地高辛标记和检测试剂盒I	11745832910	Roche		Kit	5457
	比普通DIG随机引物法标记效率高, 操作简单, 可用于Southern、Northern和斑点杂交以及菌落和噬菌体筛选。一个Kit可用于12次标记反应和24次检测反应。组成: 5×DIG-高效标记试剂; 未标记对照DNA, PBR328; DNA稀释液; 抗-DIG-碱性***酸酶复合物; NBT/BCIP储存液; 10×封闭液; DIG定量复试纸条; DIG对照试纸条, -20℃保存。
DIG-High Prime DNA Labeling and Detection Starter Kit II 高效DNA地高辛标记和检测试剂盒II	11585614910	Roche		Kit	5598
	比普通DIG随机引物法标记效率高, 采用CSPD化学发光检测灵敏度更高。一个Kit可用于12次标记反应和24次检测反应。组成: 5×高效DIG标记复合物; 未标记对照DNA, PBR328; DNA稀释液; 抗-DIG-碱性***酸酶连接物; CSPD; 10×封闭液; DIG定量试纸条; DIG对照试纸条, NBT/BCIP储存液; -20℃保存。
DIG DNA Labeling Kit 地高辛DNA标记试剂盒	11175033910	Roche		40T	7753
	随机引物扩增法以DIG-11-dUTP标记DNA; DIG标记探针可用于各种膜杂交。
DIG Nucleic acid Detection Kit 地高辛核酸检测试剂盒	11175041910	Roche		Kit	5165
	NBT/BCIP显色法检测, 灵敏度可达0.1pg同源DNA; 可检测40张100cm2杂交膜。-20℃保存。
DIG Oligonucleotide 3’-End Labeling Kit 寡核苷酸3’末端地高辛标记试剂盒	3353575910	Roche		25T	7432
	通过末端转移酶以DIG-11-ddUTP标记14-100bp的寡核苷酸探针3’端用于各种膜杂交和原位杂交。每个反应可标记100pmol的寡聚核苷酸。
DIG Oligonucleotide Tailing Kit 寡核苷酸加尾地高辛标记试剂盒	3353583910	Roche		25 T	6266
	通过末端转移酶以DIG-11-ddUTP在寡核苷酸探针3’端加一平均长度50bp(10-100bp)的寡核苷酸, 用于DNA/RNA膜杂交和原位杂交。每个反应可标记100pmol的寡聚核苷酸。
DIG RNA Labeling Kit (SP6/T7) 地高辛RNA标记试剂盒(SP6/T7)	11175025910	Roche	2´10T		7592
	由SP6或T7 RNA聚合酶通过转录反应过程标记RNA, 1mg模板可产生约10mg标记RNA用于杂交。-20℃保存。组成: pSPT18 DNA, pSPT19 DNA, 对照DNA1, 对照DNA2, DIG标记对照RNA, NTR标记混合物, 转录缓冲液, RNase抑制剂, 无RNase的DNase I, SP6 RNA聚合酶, T7 RNA聚合酶。