• Ultra high purity 5-Hydroxymethyl dCTP; >99% 

trisphosphate by HPLC 

 

• Readily incorporated into PCR amplicons with ZymoTaq™ or 

other DNA polymerases. 

 

• Free of endo-, exodeoxyribonuclease, ribonuclease, 

phosphatase and nicking activities 

 

 

Description: 

5-Hydroxymethyl dCTP from Zymo Research is of ultra high purity and 

can be used to generate DNA by PCR using ZymoTaq™ or other DNA 

polymerases. 

 

 

 

 

5-Hydroxymethylcytosine 

 

 

Applications: 

PCR amplicons containing 5-hydroxymethylcytosine are useful in a 

variety of applications such as: 

 

• The study of 5-hydroxymethylcytosine binding/interacting 

proteins 

 

• Define methods that distinguish between 5-methylcytosine 

and 5-hydroxymethylcytosine. 

 

Concentration: 10 µmol/100 µl as a [100 mM] solution. 

 

Formula: C11H14N3O14P3Li4 

 

Molecular Weight: 520.918 g/mol 

 

Storage Condition: 5-Hydroxymethyl dCTP is guaranteed for 12 

months at -20°C. Avoid multiple freeze/thaw cycles. For long-term 

storage, aliquoting is recommended with storage at -80°C.